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Loading and Protection of Hydrophilic Molecules into Liposome-Templated Polyelectrolyte Nanocapsules

Cuomo, Francesca; Ceglie, Andrea; Piludu, Marco; Miguel, Maria G.; Lindman, Björn LU and Lopez, Francesco (2014) In Langmuir 30(27). p.7993-7999
Abstract
Compartmentalized systems produced via the layer-by-layer (LbL) self-assembly method have been produced by alternatively depositing alginate and chitosan layers onto cores of liposomes. The combination of dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) techniques provides detailed information on the stability, dimensions, charge, and wall thickness of these polyelectrolyte globules. TEM microphotographs demonstrate the presence of nanocapsules with an average diameter of below 300 nm and with a polyelectrolyte wall thickness of about 20 nm. The possibility of encapsulating and releasing molecules from this type of nanocapsule was demonstrated by loading FITC-dextrans of different molecular weights... (More)
Compartmentalized systems produced via the layer-by-layer (LbL) self-assembly method have been produced by alternatively depositing alginate and chitosan layers onto cores of liposomes. The combination of dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) techniques provides detailed information on the stability, dimensions, charge, and wall thickness of these polyelectrolyte globules. TEM microphotographs demonstrate the presence of nanocapsules with an average diameter of below 300 nm and with a polyelectrolyte wall thickness of about 20 nm. The possibility of encapsulating and releasing molecules from this type of nanocapsule was demonstrated by loading FITC-dextrans of different molecular weights in the liposome system. The release of the loaded molecules from the nanocapsule was demonstrated after liposome core dissolution. Even at low molecular weight (20 kDa), the nanocapsules appear to be appropriate for prolonged molecule compartmentalization and protection. By means of the Ritger-Peppas model, non-Fickian transport behavior was detected for the diffusion of dextran through the polyelectrolyte wall. Values of the diffusion coefficient were calculated and yield useful information regarding chitosan/alginate hollow nanocapsules as drug-delivery systems. The influence of the pH on the release properties was also considered. The results indicate that vesicle-templated hollow polyelectrolyte nanocapsules show great potential as novel controllable drug-delivery devices for biomedical and biotechnological applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
30
issue
27
pages
7993 - 7999
publisher
The American Chemical Society
external identifiers
  • wos:000339229000009
  • scopus:84904409367
ISSN
0743-7463
DOI
10.1021/la501978u
language
English
LU publication?
yes
id
140cf536-56c0-40b3-8297-585d270f32c4 (old id 4592793)
date added to LUP
2014-09-05 10:57:36
date last changed
2017-07-02 03:16:39
@article{140cf536-56c0-40b3-8297-585d270f32c4,
  abstract     = {Compartmentalized systems produced via the layer-by-layer (LbL) self-assembly method have been produced by alternatively depositing alginate and chitosan layers onto cores of liposomes. The combination of dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) techniques provides detailed information on the stability, dimensions, charge, and wall thickness of these polyelectrolyte globules. TEM microphotographs demonstrate the presence of nanocapsules with an average diameter of below 300 nm and with a polyelectrolyte wall thickness of about 20 nm. The possibility of encapsulating and releasing molecules from this type of nanocapsule was demonstrated by loading FITC-dextrans of different molecular weights in the liposome system. The release of the loaded molecules from the nanocapsule was demonstrated after liposome core dissolution. Even at low molecular weight (20 kDa), the nanocapsules appear to be appropriate for prolonged molecule compartmentalization and protection. By means of the Ritger-Peppas model, non-Fickian transport behavior was detected for the diffusion of dextran through the polyelectrolyte wall. Values of the diffusion coefficient were calculated and yield useful information regarding chitosan/alginate hollow nanocapsules as drug-delivery systems. The influence of the pH on the release properties was also considered. The results indicate that vesicle-templated hollow polyelectrolyte nanocapsules show great potential as novel controllable drug-delivery devices for biomedical and biotechnological applications.},
  author       = {Cuomo, Francesca and Ceglie, Andrea and Piludu, Marco and Miguel, Maria G. and Lindman, Björn and Lopez, Francesco},
  issn         = {0743-7463},
  language     = {eng},
  number       = {27},
  pages        = {7993--7999},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Loading and Protection of Hydrophilic Molecules into Liposome-Templated Polyelectrolyte Nanocapsules},
  url          = {http://dx.doi.org/10.1021/la501978u},
  volume       = {30},
  year         = {2014},
}