Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells
(2001) In Experimental Neurology 170(2). p.25-317- Abstract
We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and... (More)
We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and functional properties characteristic of dopamine neurons in culture. These precursor cells might serve as a useful source of human dopamine neurons for studying the development and degeneration of human dopamine neurons and may further serve as a continuous, on-demand source of cells for therapeutic transplantation in patients with Parkinson's disease.
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- author
- Storch, A. ; Paul, G LU ; Csete, M ; Boehm, B. O. ; Carvey, P M ; Kupsch, A and Schwarz, J
- publishing date
- 2001-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Biomarkers, Cell Culture Techniques, Cell Differentiation, Cell Division, Cells, Cultured, Dopamine, Embryo, Mammalian, Epidermal Growth Factor, Fetus, Fibroblast Growth Factor 2, Gestational Age, Glial Cell Line-Derived Neurotrophic Factor, Growth Inhibitors, Humans, Interleukin-1, Interleukin-11, Interleukin-6, Kinetics, Leukemia Inhibitory Factor, Lymphokines, Mesencephalon, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Oxygen, Prosencephalon, Stem Cells, Time Factors, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
- in
- Experimental Neurology
- volume
- 170
- issue
- 2
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0034871846
- pmid:11476598
- ISSN
- 0014-4886
- DOI
- 10.1006/exnr.2001.7706
- language
- English
- LU publication?
- no
- id
- 45ab104d-b981-4b64-b1ae-a70f9265c162
- date added to LUP
- 2017-05-18 12:44:38
- date last changed
- 2024-07-07 18:18:54
@article{45ab104d-b981-4b64-b1ae-a70f9265c162, abstract = {{<p>We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and functional properties characteristic of dopamine neurons in culture. These precursor cells might serve as a useful source of human dopamine neurons for studying the development and degeneration of human dopamine neurons and may further serve as a continuous, on-demand source of cells for therapeutic transplantation in patients with Parkinson's disease.</p>}}, author = {{Storch, A. and Paul, G and Csete, M and Boehm, B. O. and Carvey, P M and Kupsch, A and Schwarz, J}}, issn = {{0014-4886}}, keywords = {{Biomarkers; Cell Culture Techniques; Cell Differentiation; Cell Division; Cells, Cultured; Dopamine; Embryo, Mammalian; Epidermal Growth Factor; Fetus; Fibroblast Growth Factor 2; Gestational Age; Glial Cell Line-Derived Neurotrophic Factor; Growth Inhibitors; Humans; Interleukin-1; Interleukin-11; Interleukin-6; Kinetics; Leukemia Inhibitory Factor; Lymphokines; Mesencephalon; Nerve Growth Factors; Nerve Tissue Proteins; Neurons; Oxygen; Prosencephalon; Stem Cells; Time Factors; Tyrosine 3-Monooxygenase; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.}}, language = {{eng}}, number = {{2}}, pages = {{25--317}}, publisher = {{Elsevier}}, series = {{Experimental Neurology}}, title = {{Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells}}, url = {{http://dx.doi.org/10.1006/exnr.2001.7706}}, doi = {{10.1006/exnr.2001.7706}}, volume = {{170}}, year = {{2001}}, }