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Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells

Storch, A.; Paul, G LU ; Csete, M; Boehm, B. O.; Carvey, P M; Kupsch, A and Schwarz, J (2001) In Experimental Neurology 170(2). p.25-317
Abstract

We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and... (More)

We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and functional properties characteristic of dopamine neurons in culture. These precursor cells might serve as a useful source of human dopamine neurons for studying the development and degeneration of human dopamine neurons and may further serve as a continuous, on-demand source of cells for therapeutic transplantation in patients with Parkinson's disease.

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publishing date
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Contribution to journal
publication status
published
keywords
Biomarkers, Cell Culture Techniques, Cell Differentiation, Cell Division, Cells, Cultured, Dopamine, Embryo, Mammalian, Epidermal Growth Factor, Fetus, Fibroblast Growth Factor 2, Gestational Age, Glial Cell Line-Derived Neurotrophic Factor, Growth Inhibitors, Humans, Interleukin-1, Interleukin-11, Interleukin-6, Kinetics, Leukemia Inhibitory Factor, Lymphokines, Mesencephalon, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Oxygen, Prosencephalon, Stem Cells, Time Factors, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
in
Experimental Neurology
volume
170
issue
2
pages
9 pages
publisher
Academic Press
external identifiers
  • scopus:0034871846
ISSN
0014-4886
DOI
language
English
LU publication?
no
id
45ab104d-b981-4b64-b1ae-a70f9265c162
date added to LUP
2017-05-18 12:44:38
date last changed
2018-05-29 12:06:26
@article{45ab104d-b981-4b64-b1ae-a70f9265c162,
  abstract     = {<p>We report on generation of dopamine neurons from long-term cultures of human fetal mesencephalic precursor cells. These CNS precursor cells were successfully expanded in vitro using the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3% atmospheric oxygen resulted in higher cellular yields than room air. Following incubation in differentiation media containing interleukin (IL)-1b (IL-1b), IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine neurons. The TH immunoreactive cells exhibited morphological and functional properties characteristic of dopamine neurons in culture. These precursor cells might serve as a useful source of human dopamine neurons for studying the development and degeneration of human dopamine neurons and may further serve as a continuous, on-demand source of cells for therapeutic transplantation in patients with Parkinson's disease.</p>},
  author       = {Storch, A. and Paul, G and Csete, M and Boehm, B. O. and Carvey, P M and Kupsch, A and Schwarz, J},
  issn         = {0014-4886},
  keyword      = {Biomarkers,Cell Culture Techniques,Cell Differentiation,Cell Division,Cells, Cultured,Dopamine,Embryo, Mammalian,Epidermal Growth Factor,Fetus,Fibroblast Growth Factor 2,Gestational Age,Glial Cell Line-Derived Neurotrophic Factor,Growth Inhibitors,Humans,Interleukin-1,Interleukin-11,Interleukin-6,Kinetics,Leukemia Inhibitory Factor,Lymphokines,Mesencephalon,Nerve Growth Factors,Nerve Tissue Proteins,Neurons,Oxygen,Prosencephalon,Stem Cells,Time Factors,Tyrosine 3-Monooxygenase,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.},
  language     = {eng},
  number       = {2},
  pages        = {25--317},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells},
  url          = {http://dx.doi.org/},
  volume       = {170},
  year         = {2001},
}