Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format

Hanora, Amro LU ; Bernaudat, Florent LU ; Plieva, Fatima LU ; Dainiak, Maria LU ; Bülow, Leif LU ; Galaev, Igor LU and Mattiasson, Bo LU (2005) In Journal of Chromatography A 1087(1-2). p.38-44
Abstract
A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding peptide tags library obtained from E. coli against different metal ions. From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries. (c) 2005 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1087
issue
1-2
pages
38 - 44
publisher
Elsevier
external identifiers
  • pmid:16130695
  • wos:000231185600006
  • scopus:23044450523
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.04.029
language
English
LU publication?
yes
id
45b57889-4542-48ce-b986-66f108ee7c47 (old id 152619)
date added to LUP
2016-04-01 15:45:17
date last changed
2022-04-22 17:11:32
@article{45b57889-4542-48ce-b986-66f108ee7c47,
  abstract     = {{A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding peptide tags library obtained from E. coli against different metal ions. From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries. (c) 2005 Elsevier B.V. All rights reserved.}},
  author       = {{Hanora, Amro and Bernaudat, Florent and Plieva, Fatima and Dainiak, Maria and Bülow, Leif and Galaev, Igor and Mattiasson, Bo}},
  issn         = {{0021-9673}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{38--44}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format}},
  url          = {{http://dx.doi.org/10.1016/j.chroma.2005.04.029}},
  doi          = {{10.1016/j.chroma.2005.04.029}},
  volume       = {{1087}},
  year         = {{2005}},
}