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Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine

Lebogang, Lesedi LU ; Mattiasson, Bo LU and Hedström, Martin LU (2014) In Mikrochimica Acta 181(9-10). p.1009-1017
Abstract
We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the... (More)
We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capacitive biosensor, Microcystins, Adda-specific antibodies, M., aeruginosa, Real-time analysis
in
Mikrochimica Acta
volume
181
issue
9-10
pages
1009 - 1017
publisher
Springer
external identifiers
  • wos:000338317000018
  • scopus:84903125269
ISSN
1436-5073
DOI
10.1007/s00604-014-1199-4
language
English
LU publication?
yes
id
4d121b40-a036-426f-ae3d-8978c0a70c4e (old id 4602819)
date added to LUP
2014-09-04 13:05:07
date last changed
2017-10-29 03:05:24
@article{4d121b40-a036-426f-ae3d-8978c0a70c4e,
  abstract     = {We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da).},
  author       = {Lebogang, Lesedi and Mattiasson, Bo and Hedström, Martin},
  issn         = {1436-5073},
  keyword      = {Capacitive biosensor,Microcystins,Adda-specific antibodies,M.,aeruginosa,Real-time analysis},
  language     = {eng},
  number       = {9-10},
  pages        = {1009--1017},
  publisher    = {Springer},
  series       = {Mikrochimica Acta},
  title        = {Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine},
  url          = {http://dx.doi.org/10.1007/s00604-014-1199-4},
  volume       = {181},
  year         = {2014},
}