Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells
(2020) In Nature Communications 11(1).- Abstract
In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency.... (More)
In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and -521 without the need for manual isolation.
(Less)
- author
- publishing date
- 2020-03-30
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Biomarkers/metabolism, CD56 Antigen, Cell Differentiation/physiology, Embryonic Stem Cells, Epithelial Cells/metabolism, Humans, Laminin/genetics, Macular Degeneration/metabolism, Neurons/metabolism, Rabbits, Retinal Pigment Epithelium/metabolism, Retinal Pigments/metabolism
- in
- Nature Communications
- volume
- 11
- issue
- 1
- pages
- 15 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:32231223
- scopus:85082731796
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-020-15326-5
- language
- English
- LU publication?
- no
- id
- 4603111f-191e-46f1-8ef2-ca0799066d36
- date added to LUP
- 2021-08-09 14:28:05
- date last changed
- 2024-04-06 06:24:39
@article{4603111f-191e-46f1-8ef2-ca0799066d36, abstract = {{<p>In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and -521 without the need for manual isolation.</p>}}, author = {{Plaza Reyes, Alvaro and Petrus-Reurer, Sandra and Padrell Sánchez, Sara and Kumar, Pankaj and Douagi, Iyadh and Bartuma, Hammurabi and Aronsson, Monica and Westman, Sofie and Lardner, Emma and André, Helder and Falk, Anna and Nandrot, Emeline F and Kvanta, Anders and Lanner, Fredrik}}, issn = {{2041-1723}}, keywords = {{Animals; Biomarkers/metabolism; CD56 Antigen; Cell Differentiation/physiology; Embryonic Stem Cells; Epithelial Cells/metabolism; Humans; Laminin/genetics; Macular Degeneration/metabolism; Neurons/metabolism; Rabbits; Retinal Pigment Epithelium/metabolism; Retinal Pigments/metabolism}}, language = {{eng}}, month = {{03}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells}}, url = {{https://lup.lub.lu.se/search/files/101032682/Identification_of_cell_surface_markers.pdf}}, doi = {{10.1038/s41467-020-15326-5}}, volume = {{11}}, year = {{2020}}, }