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Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples

Tende, Talatu LU ; Hansson, Bengt LU ; Ottosson, Ulf LU and Bensch, Staffan LU (2014) In Current Zoology 60(3). p.351-358
Abstract
Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that employed different media for the storage of faeces, although patterns of success were not fully consistent among different media, ethanol storage was scored... (More)
Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that employed different media for the storage of faeces, although patterns of success were not fully consistent among different media, ethanol storage was scored highest in the majority of these tests. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amplification success, Faecal sample DNA, Lion Panthera leo, PCR, Preservation medium
in
Current Zoology
volume
60
issue
3
pages
351 - 358
publisher
Current Zoology
external identifiers
  • wos:000338119400004
  • scopus:84903135826
ISSN
1674-5507
project
BECC
Conservation of threatened populations
language
English
LU publication?
yes
id
ed84f69f-77b1-4c89-ae18-7461d3f1246c (old id 4608688)
date added to LUP
2014-09-04 11:03:58
date last changed
2017-09-24 03:55:52
@article{ed84f69f-77b1-4c89-ae18-7461d3f1246c,
  abstract     = {Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that employed different media for the storage of faeces, although patterns of success were not fully consistent among different media, ethanol storage was scored highest in the majority of these tests.},
  author       = {Tende, Talatu and Hansson, Bengt and Ottosson, Ulf and Bensch, Staffan},
  issn         = {1674-5507},
  keyword      = {Amplification success,Faecal sample DNA,Lion Panthera leo,PCR,Preservation medium},
  language     = {eng},
  number       = {3},
  pages        = {351--358},
  publisher    = {Current Zoology},
  series       = {Current Zoology},
  title        = {Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples},
  volume       = {60},
  year         = {2014},
}