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A Highly UV-transparent Fused Silica Biochip for Sensitive Hepatotoxicity Testing by Autofluorescence

Fritzsche, Michael; Fritzsche, Joachim; Tegenfeldt, Jonas LU and Mandenius, Carl-Fredrik (2014) In Biochip Journal 8(2). p.115-121
Abstract
Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The... (More)
Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The autofluorescence from the.liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autofluorescence, Drug testing, Microfluidic chip, Fused silica, In, vitro toxicity, HepG2 cell line
in
Biochip Journal
volume
8
issue
2
pages
115 - 121
publisher
Korean BioChip Society (KBCS)
external identifiers
  • wos:000337717900006
  • scopus:84903519977
ISSN
2092-7843
DOI
10.1007/s13206-014-8206-z
language
English
LU publication?
yes
id
edefeb4b-59f3-40c0-ad59-e13390ccdd7d (old id 4609525)
date added to LUP
2014-08-26 10:47:24
date last changed
2017-01-01 03:18:44
@article{edefeb4b-59f3-40c0-ad59-e13390ccdd7d,
  abstract     = {Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The autofluorescence from the.liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data.},
  author       = {Fritzsche, Michael and Fritzsche, Joachim and Tegenfeldt, Jonas and Mandenius, Carl-Fredrik},
  issn         = {2092-7843},
  keyword      = {Autofluorescence,Drug testing,Microfluidic chip,Fused silica,In,vitro toxicity,HepG2 cell line},
  language     = {eng},
  number       = {2},
  pages        = {115--121},
  publisher    = {Korean BioChip Society (KBCS)},
  series       = {Biochip Journal},
  title        = {A Highly UV-transparent Fused Silica Biochip for Sensitive Hepatotoxicity Testing by Autofluorescence},
  url          = {http://dx.doi.org/10.1007/s13206-014-8206-z},
  volume       = {8},
  year         = {2014},
}