Advanced

Identification of molecular mechanisms used by Finegoldia magna to penetrate and colonise human skin.

Murphy, Elizabeth LU ; Mörgelin, Matthias LU ; Reinhardt, Dieter P; Olin, Anders LU ; Björck, Lars LU and Frick, Inga-Maria LU (2014) In Molecular Microbiology 94(2). p.403-417
Abstract
Finegoldia magna is a Gram-positive anaerobic commensal of the human skin microbiota, but also known to act as an opportunistic pathogen. Two primary virulence factors of F. magna are the subtilisin-like extracellular serine protease SufA and the adhesive protein FAF. This study examines the molecular mechanisms F. magna uses when colonising or establishing an infection in the skin. FAF was found to be essential in the initial adherence of F. magna to human skin biopsies. In the upper layers of the epidermis FAF mediates adhesion through binding to galectin-7 - a keratinocyte cell marker. Once the bacteria moved deeper into the skin to the basement membrane layer, SufA was found to degrade collagen IV which forms the backbone structure of... (More)
Finegoldia magna is a Gram-positive anaerobic commensal of the human skin microbiota, but also known to act as an opportunistic pathogen. Two primary virulence factors of F. magna are the subtilisin-like extracellular serine protease SufA and the adhesive protein FAF. This study examines the molecular mechanisms F. magna uses when colonising or establishing an infection in the skin. FAF was found to be essential in the initial adherence of F. magna to human skin biopsies. In the upper layers of the epidermis FAF mediates adhesion through binding to galectin-7 - a keratinocyte cell marker. Once the bacteria moved deeper into the skin to the basement membrane layer, SufA was found to degrade collagen IV which forms the backbone structure of the basement membrane. It also degraded collagen V, whereby F. magna could reach deeper dermal tissue sites. In the dermis, FAF interacts with collagen V and fibrillin, which presumably helps the bacteria to establish infection in this area. The findings of this study paint a clear picture of how F. magna interacts with human skin and explain how it is such a successful opportunistic pathogen in chronic wounds and ulcers. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
94
issue
2
pages
403 - 417
publisher
Wiley-Blackwell
external identifiers
  • pmid:25164331
  • wos:000343755700012
  • scopus:84916634667
ISSN
1365-2958
DOI
10.1111/mmi.12773
language
English
LU publication?
yes
id
b9da69a8-e484-4e87-ade4-f1757b97f921 (old id 4613776)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25164331?dopt=Abstract
date added to LUP
2014-09-09 19:08:43
date last changed
2017-11-05 03:09:25
@article{b9da69a8-e484-4e87-ade4-f1757b97f921,
  abstract     = {Finegoldia magna is a Gram-positive anaerobic commensal of the human skin microbiota, but also known to act as an opportunistic pathogen. Two primary virulence factors of F. magna are the subtilisin-like extracellular serine protease SufA and the adhesive protein FAF. This study examines the molecular mechanisms F. magna uses when colonising or establishing an infection in the skin. FAF was found to be essential in the initial adherence of F. magna to human skin biopsies. In the upper layers of the epidermis FAF mediates adhesion through binding to galectin-7 - a keratinocyte cell marker. Once the bacteria moved deeper into the skin to the basement membrane layer, SufA was found to degrade collagen IV which forms the backbone structure of the basement membrane. It also degraded collagen V, whereby F. magna could reach deeper dermal tissue sites. In the dermis, FAF interacts with collagen V and fibrillin, which presumably helps the bacteria to establish infection in this area. The findings of this study paint a clear picture of how F. magna interacts with human skin and explain how it is such a successful opportunistic pathogen in chronic wounds and ulcers.},
  author       = {Murphy, Elizabeth and Mörgelin, Matthias and Reinhardt, Dieter P and Olin, Anders and Björck, Lars and Frick, Inga-Maria},
  issn         = {1365-2958},
  language     = {eng},
  number       = {2},
  pages        = {403--417},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Identification of molecular mechanisms used by Finegoldia magna to penetrate and colonise human skin.},
  url          = {http://dx.doi.org/10.1111/mmi.12773},
  volume       = {94},
  year         = {2014},
}