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Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate.

Wallace, Valeria LU ; Signori, Lorenzo; Li, Ying-Ying; Ask, Magnus; Bettiga, Maurizio; Porro, Danilo; Thevelein, Johan M; Branduardi, Paola; Foulquié-Moreno, María R and Gorwa-Grauslund, Marie-Francoise LU (2014) In AMB Express 4.
Abstract
Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and... (More)
Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
AMB Express
volume
4
publisher
Springer Open
external identifiers
  • pmid:25147754
  • scopus:84947046745
ISSN
2191-0855
DOI
10.1186/s13568-014-0056-5
language
English
LU publication?
yes
id
8d885d69-7aca-4b52-825d-ca67f0043203 (old id 4614174)
date added to LUP
2014-09-12 16:42:20
date last changed
2017-08-27 04:50:35
@article{8d885d69-7aca-4b52-825d-ca67f0043203,
  abstract     = {Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed.},
  articleno    = {56},
  author       = {Wallace, Valeria and Signori, Lorenzo and Li, Ying-Ying and Ask, Magnus and Bettiga, Maurizio and Porro, Danilo and Thevelein, Johan M and Branduardi, Paola and Foulquié-Moreno, María R and Gorwa-Grauslund, Marie-Francoise},
  issn         = {2191-0855},
  language     = {eng},
  publisher    = {Springer Open},
  series       = {AMB Express},
  title        = {Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate.},
  url          = {http://dx.doi.org/10.1186/s13568-014-0056-5},
  volume       = {4},
  year         = {2014},
}