CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.
(2015) In Mucosal Immunology 8(2). p.327-339- Abstract
- The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the... (More)
- The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4614296
- author
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Mucosal Immunology
- volume
- 8
- issue
- 2
- pages
- 327 - 339
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:25138666
- wos:000349681200010
- scopus:84922714497
- pmid:25138666
- ISSN
- 1933-0219
- DOI
- 10.1038/mi.2014.70
- language
- English
- LU publication?
- yes
- id
- 8312672c-817a-4aa8-b216-017b1dfdcfae (old id 4614296)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25138666?dopt=Abstract
- date added to LUP
- 2016-04-01 10:06:10
- date last changed
- 2024-12-17 04:09:26
@article{8312672c-817a-4aa8-b216-017b1dfdcfae, abstract = {{The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70.}}, author = {{Scott, C L and Bain, C C and Wright, P B and Sichien, D and Kotarsky, Knut and Persson, Emma and Luda, Katarzyna and Guilliams, M and Lambrecht, B N and Agace, William and Milling, S Wf and Mowat, Allan}}, issn = {{1933-0219}}, language = {{eng}}, number = {{2}}, pages = {{327--339}}, publisher = {{Nature Publishing Group}}, series = {{Mucosal Immunology}}, title = {{CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.}}, url = {{http://dx.doi.org/10.1038/mi.2014.70}}, doi = {{10.1038/mi.2014.70}}, volume = {{8}}, year = {{2015}}, }