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CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.

Scott, C L; Bain, C C; Wright, P B; Sichien, D; Kotarsky, Knut LU ; Persson, Emma LU ; Luda, Katarzyna LU ; Guilliams, M; Lambrecht, B N and Agace, William LU , et al. (2015) In Mucosal Immunology 8(2). p.327-339
Abstract
The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the... (More)
The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70. (Less)
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Mucosal Immunology
volume
8
issue
2
pages
327 - 339
publisher
Nature Publishing Group
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  • pmid:25138666
  • wos:000349681200010
  • scopus:84922714497
ISSN
1933-0219
DOI
10.1038/mi.2014.70
language
English
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yes
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8312672c-817a-4aa8-b216-017b1dfdcfae (old id 4614296)
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http://www.ncbi.nlm.nih.gov/pubmed/25138666?dopt=Abstract
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2014-09-07 21:39:58
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2017-10-01 03:07:34
@article{8312672c-817a-4aa8-b216-017b1dfdcfae,
  abstract     = {The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70.},
  author       = {Scott, C L and Bain, C C and Wright, P B and Sichien, D and Kotarsky, Knut and Persson, Emma and Luda, Katarzyna and Guilliams, M and Lambrecht, B N and Agace, William and Milling, S Wf and Mowat, Allan},
  issn         = {1933-0219},
  language     = {eng},
  number       = {2},
  pages        = {327--339},
  publisher    = {Nature Publishing Group},
  series       = {Mucosal Immunology},
  title        = {CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.},
  url          = {http://dx.doi.org/10.1038/mi.2014.70},
  volume       = {8},
  year         = {2015},
}