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Human oral, gut, and plaque microbiota in patients with atherosclerosis

Koren, O.; Spor, A.; Felin, J.; Fåk, Frida LU ; Stombaugh, J.; Tremaroli, V.; Behre, C. J.; Knight, R.; Fagerberg, B. and Ley, R. E., et al. (2011) In Proceedings of the National Academy of Sciences 108(Suppl. 1). p.8-4592
Abstract
Periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. Furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. Using qPCR, we show that bacterial DNA was present in the atherosclerotic plaque and that the amount of DNA correlated with the amount of leukocytes in the atherosclerotic plaque. To investigate the microbial composition of atherosclerotic plaques and test the hypothesis that the oral or gut microbiota may contribute to atherosclerosis in humans, we used 454 pyrosequencing of 16S rRNA genes to survey the bacterial diversity of atherosclerotic plaque, oral, and... (More)
Periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. Furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. Using qPCR, we show that bacterial DNA was present in the atherosclerotic plaque and that the amount of DNA correlated with the amount of leukocytes in the atherosclerotic plaque. To investigate the microbial composition of atherosclerotic plaques and test the hypothesis that the oral or gut microbiota may contribute to atherosclerosis in humans, we used 454 pyrosequencing of 16S rRNA genes to survey the bacterial diversity of atherosclerotic plaque, oral, and gut samples of 15 patients with atherosclerosis, and oral and gut samples of healthy controls. We identified Chryseomonas in all atherosclerotic plaque samples, and Veillonella and Streptococcus in the majority. Interestingly, the combined abundances of Veillonella and Streptococcus in atherosclerotic plaques correlated with their abundance in the oral cavity. Moreover, several additional bacterial phylotypes were common to the atherosclerotic plaque and oral or gut samples within the same individual. Interestingly, several bacterial taxa in the oral cavity and the gut correlated with plasma cholesterol levels. Taken together, our findings suggest that bacteria from the oral cavity, and perhaps even the gut, may correlate with disease markers of atherosclerosis. (Less)
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Plaque, Phylogeny, Mouth/*microbiology, Molecular Sequence Data, Metagenome/*genetics, Male, Humans, Gastrointestinal Tract/*microbiology, Female, Cluster Analysis, Base Sequence, Bacteria/*genetics, Aged, Atherosclerosis/*microbiology, Atherosclerotic/*microbiology, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Sweden
in
Proceedings of the National Academy of Sciences
volume
108
issue
Suppl. 1
pages
8 - 4592
publisher
National Acad Sciences
external identifiers
  • scopus:79952758083
ISSN
1091-6490
DOI
10.1073/pnas.1011383107
language
English
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no
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be77a0ac-494d-46be-80fb-1e74bb40f631 (old id 4616714)
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2016-01-27 12:38:01
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2017-11-19 04:16:51
@article{be77a0ac-494d-46be-80fb-1e74bb40f631,
  abstract     = {Periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. Furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. Using qPCR, we show that bacterial DNA was present in the atherosclerotic plaque and that the amount of DNA correlated with the amount of leukocytes in the atherosclerotic plaque. To investigate the microbial composition of atherosclerotic plaques and test the hypothesis that the oral or gut microbiota may contribute to atherosclerosis in humans, we used 454 pyrosequencing of 16S rRNA genes to survey the bacterial diversity of atherosclerotic plaque, oral, and gut samples of 15 patients with atherosclerosis, and oral and gut samples of healthy controls. We identified Chryseomonas in all atherosclerotic plaque samples, and Veillonella and Streptococcus in the majority. Interestingly, the combined abundances of Veillonella and Streptococcus in atherosclerotic plaques correlated with their abundance in the oral cavity. Moreover, several additional bacterial phylotypes were common to the atherosclerotic plaque and oral or gut samples within the same individual. Interestingly, several bacterial taxa in the oral cavity and the gut correlated with plasma cholesterol levels. Taken together, our findings suggest that bacteria from the oral cavity, and perhaps even the gut, may correlate with disease markers of atherosclerosis.},
  author       = {Koren, O. and Spor, A. and Felin, J. and Fåk, Frida and Stombaugh, J. and Tremaroli, V. and Behre, C. J. and Knight, R. and Fagerberg, B. and Ley, R. E. and Backhed, F.},
  issn         = {1091-6490},
  keyword      = {Plaque,Phylogeny,Mouth/*microbiology,Molecular Sequence Data,Metagenome/*genetics,Male,Humans,Gastrointestinal Tract/*microbiology,Female,Cluster Analysis,Base Sequence,Bacteria/*genetics,Aged,Atherosclerosis/*microbiology,Atherosclerotic/*microbiology,Polymerase Chain Reaction,RNA,Ribosomal,16S/genetics,Sequence Analysis,DNA,Species Specificity,Sweden},
  language     = {eng},
  number       = {Suppl. 1},
  pages        = {8--4592},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Human oral, gut, and plaque microbiota in patients with atherosclerosis},
  url          = {http://dx.doi.org/10.1073/pnas.1011383107},
  volume       = {108},
  year         = {2011},
}