Advanced

Lactobacillus reuteri prevents diet-induced obesity, but not atherosclerosis, in a strain dependent fashion in Apoe-/- mice

Fåk, Frida LU and Backhed, F. (2012) In PLoS ONE 7.
Abstract
OBJECTIVE: To investigate whether the specific strains of Lactobacillus reuteri modulates the metabolic syndrome in Apoe-/- mice. METHODS: 8 week-old Apoe-/- mice were subdivided into four groups who received either L. reuteri ATCC PTA 4659 (ATCC), DSM 17938 (DSM), L6798, or no bacterial supplement in the drinking water for 12 weeks. The mice were fed a high-fat Western diet with 0.2% cholesterol and body weights were monitored weekly. At the end of the study, oral glucose and insulin tolerance tests were conducted. In addition, adipose and liver weights were recorded along with analyses of mRNA expression of ileal Angiopoietin-like protein 4 (Angptl4), the macrophage marker F4/80 encoded by the gene Emr1 and liver Acetyl-CoA carboxylase 1... (More)
OBJECTIVE: To investigate whether the specific strains of Lactobacillus reuteri modulates the metabolic syndrome in Apoe-/- mice. METHODS: 8 week-old Apoe-/- mice were subdivided into four groups who received either L. reuteri ATCC PTA 4659 (ATCC), DSM 17938 (DSM), L6798, or no bacterial supplement in the drinking water for 12 weeks. The mice were fed a high-fat Western diet with 0.2% cholesterol and body weights were monitored weekly. At the end of the study, oral glucose and insulin tolerance tests were conducted. In addition, adipose and liver weights were recorded along with analyses of mRNA expression of ileal Angiopoietin-like protein 4 (Angptl4), the macrophage marker F4/80 encoded by the gene Emr1 and liver Acetyl-CoA carboxylase 1 (Acc1), Fatty acid synthase (Fas) and Carnitine palmitoyltransferase 1a (Cpt1a). Atherosclerosis was assessed in the aortic root region of the heart. RESULTS AND CONCLUSIONS: Mice receiving L. reuteri ATCC gained significantly less body weight than the control mice, whereas the L6798 mice gained significantly more. Adipose and liver weights were also reduced in the ATCC group. Serum insulin levels were lower in the ATCC group, but no significant effects were observed in the glucose or insulin tolerance tests. Lipogenic genes in the liver were not altered by any of the bacterial treatments, however, increased expression of Cpt1a was found in the ATCC group, indicating increased beta-oxidation. Correspondingly, the liver trended towards having lower fat content. There were no effects on inflammatory markers, blood cholesterol or atherosclerosis. In conclusion, the probiotic L. reuteri strain ATCC PTA 4659 partly prevented diet-induced obesity, possibly via a previously unknown mechanism of inducing liver expression of Cpt1a. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactobacillus reuteri/*physiology, Insulin Resistance, Inflammation/etiology/metabolism/microbiology, Humans, Fatty Liver/etiology/metabolism/microbiology/prevention & control, High-Fat/*adverse effects, Diet, Atherosclerosis/etiology/metabolism/*microbiology/*prevention & control, Animals, Apolipoproteins E/*deficiency, Mice, Obesity/etiology/metabolism/*microbiology/*prevention & control, Phenotype, Probiotics/pharmacology, Species Specificity
in
PLoS ONE
volume
7
publisher
Public Library of Science
external identifiers
  • scopus:84867287315
ISSN
1932-6203
DOI
10.1371/journal.pone.0046837
language
English
LU publication?
no
id
ff713830-a54d-4032-9dd8-6e0b8563a5d8 (old id 4616749)
date added to LUP
2016-01-27 15:30:05
date last changed
2017-10-08 04:34:28
@article{ff713830-a54d-4032-9dd8-6e0b8563a5d8,
  abstract     = {OBJECTIVE: To investigate whether the specific strains of Lactobacillus reuteri modulates the metabolic syndrome in Apoe-/- mice. METHODS: 8 week-old Apoe-/- mice were subdivided into four groups who received either L. reuteri ATCC PTA 4659 (ATCC), DSM 17938 (DSM), L6798, or no bacterial supplement in the drinking water for 12 weeks. The mice were fed a high-fat Western diet with 0.2% cholesterol and body weights were monitored weekly. At the end of the study, oral glucose and insulin tolerance tests were conducted. In addition, adipose and liver weights were recorded along with analyses of mRNA expression of ileal Angiopoietin-like protein 4 (Angptl4), the macrophage marker F4/80 encoded by the gene Emr1 and liver Acetyl-CoA carboxylase 1 (Acc1), Fatty acid synthase (Fas) and Carnitine palmitoyltransferase 1a (Cpt1a). Atherosclerosis was assessed in the aortic root region of the heart. RESULTS AND CONCLUSIONS: Mice receiving L. reuteri ATCC gained significantly less body weight than the control mice, whereas the L6798 mice gained significantly more. Adipose and liver weights were also reduced in the ATCC group. Serum insulin levels were lower in the ATCC group, but no significant effects were observed in the glucose or insulin tolerance tests. Lipogenic genes in the liver were not altered by any of the bacterial treatments, however, increased expression of Cpt1a was found in the ATCC group, indicating increased beta-oxidation. Correspondingly, the liver trended towards having lower fat content. There were no effects on inflammatory markers, blood cholesterol or atherosclerosis. In conclusion, the probiotic L. reuteri strain ATCC PTA 4659 partly prevented diet-induced obesity, possibly via a previously unknown mechanism of inducing liver expression of Cpt1a.},
  author       = {Fåk, Frida and Backhed, F.},
  issn         = {1932-6203},
  keyword      = {Lactobacillus reuteri/*physiology,Insulin Resistance,Inflammation/etiology/metabolism/microbiology,Humans,Fatty Liver/etiology/metabolism/microbiology/prevention & control,High-Fat/*adverse effects,Diet,Atherosclerosis/etiology/metabolism/*microbiology/*prevention & control,Animals,Apolipoproteins E/*deficiency,Mice,Obesity/etiology/metabolism/*microbiology/*prevention & control,Phenotype,Probiotics/pharmacology,Species Specificity},
  language     = {eng},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Lactobacillus reuteri prevents diet-induced obesity, but not atherosclerosis, in a strain dependent fashion in Apoe-/- mice},
  url          = {http://dx.doi.org/10.1371/journal.pone.0046837},
  volume       = {7},
  year         = {2012},
}