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A method for extracting plant roots from soil which facilitates rapid sample processing without compromising measurement accuracy

Metcalfe, Dan LU ; Williams, M.; Aragao, L. E. O. C.; da Costa, A. C. L.; de Almeida, S. S.; Braga, A. P.; Goncalves, P. H. L. and Silva Junior, J. de Athaydes (2007) In New Phytologist 174(3). p.697-703
Abstract
This study evaluates a novel method for extracting roots from soil samples and applies it to estimate standing crop root mass (+/- confidence intervals) in an eastern Amazon rainforest. Roots were manually extracted from soil cores over a period of 40 min, which was split into 10 min time intervals. The pattern of cumulative extraction over time was used to predict root extraction beyond 40 min. A maximum-likelihood approach was used to calculate confidence intervals. The temporal prediction method added 21-32% to initial estimates of standing crop root mass. According to predictions, complete manual root extraction from 18 samples would have taken c. 239 h, compared with 12 h using the prediction method. Uncertainties (percentage... (More)
This study evaluates a novel method for extracting roots from soil samples and applies it to estimate standing crop root mass (+/- confidence intervals) in an eastern Amazon rainforest. Roots were manually extracted from soil cores over a period of 40 min, which was split into 10 min time intervals. The pattern of cumulative extraction over time was used to predict root extraction beyond 40 min. A maximum-likelihood approach was used to calculate confidence intervals. The temporal prediction method added 21-32% to initial estimates of standing crop root mass. According to predictions, complete manual root extraction from 18 samples would have taken c. 239 h, compared with 12 h using the prediction method. Uncertainties (percentage difference between mean, and 10th and 90th percentiles) introduced by the prediction method were small (12-15%), compared with uncertainties caused by spatial variation in root mass (72-191%, for nine samples per plot surveyed). This method provides a way of increasing the number of root samples processed per unit time, without compromising measurement accuracy. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amazon tropical rainforest, maximum-likelihood approach, methodological, evaluation, root sampling method, standing crop root mass, temporal, prediction method
in
New Phytologist
volume
174
issue
3
pages
697 - 703
publisher
Wiley-Blackwell
external identifiers
  • wos:000245745500024
  • scopus:34247343348
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2007.02032.x
language
English
LU publication?
no
id
0a274913-8d69-47d6-a263-c566e0ccf2c3 (old id 4644563)
date added to LUP
2014-09-23 15:09:35
date last changed
2017-08-27 03:57:28
@article{0a274913-8d69-47d6-a263-c566e0ccf2c3,
  abstract     = {This study evaluates a novel method for extracting roots from soil samples and applies it to estimate standing crop root mass (+/- confidence intervals) in an eastern Amazon rainforest. Roots were manually extracted from soil cores over a period of 40 min, which was split into 10 min time intervals. The pattern of cumulative extraction over time was used to predict root extraction beyond 40 min. A maximum-likelihood approach was used to calculate confidence intervals. The temporal prediction method added 21-32% to initial estimates of standing crop root mass. According to predictions, complete manual root extraction from 18 samples would have taken c. 239 h, compared with 12 h using the prediction method. Uncertainties (percentage difference between mean, and 10th and 90th percentiles) introduced by the prediction method were small (12-15%), compared with uncertainties caused by spatial variation in root mass (72-191%, for nine samples per plot surveyed). This method provides a way of increasing the number of root samples processed per unit time, without compromising measurement accuracy.},
  author       = {Metcalfe, Dan and Williams, M. and Aragao, L. E. O. C. and da Costa, A. C. L. and de Almeida, S. S. and Braga, A. P. and Goncalves, P. H. L. and Silva Junior, J. de Athaydes},
  issn         = {1469-8137},
  keyword      = {Amazon tropical rainforest,maximum-likelihood approach,methodological,evaluation,root sampling method,standing crop root mass,temporal,prediction method},
  language     = {eng},
  number       = {3},
  pages        = {697--703},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {A method for extracting plant roots from soil which facilitates rapid sample processing without compromising measurement accuracy},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2007.02032.x},
  volume       = {174},
  year         = {2007},
}