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Studies of Electrocatalytic Reactions of NADH at Mediator Modified Electrodes and of H2O2 at Peroxidase Modified Electrodes

Munteanu, Florentina LU (2002)
Abstract (Swedish)
Popular Abstract in Swedish

Många biokemiska reaktioner har höga aktiveringsenergier och utan de katalyserande enzymerna skulle de flesta ämnes- omsättningsprocesser fortskrida alldeles för långsamt för att uppehålla liv. Enzymer kallas ofta för biokatalysatorer eftersom de verkar katalytiskt, dvs påskyndar kemiska reaktioner utan att själva förändras. De finns i en enorm variation eftersom vart och ett oftast bara är inblandat i en reaktion. Enzymerna behöver bara finnas i ytterst små mängder för att påverka hastigheten för en reaktion.



I denna avhandling elektrokemiska biosensorer använts och i avhandlingen ingår studier av direkt och medierad elektronöverföring mellan immobiliserade enzymer... (More)
Popular Abstract in Swedish

Många biokemiska reaktioner har höga aktiveringsenergier och utan de katalyserande enzymerna skulle de flesta ämnes- omsättningsprocesser fortskrida alldeles för långsamt för att uppehålla liv. Enzymer kallas ofta för biokatalysatorer eftersom de verkar katalytiskt, dvs påskyndar kemiska reaktioner utan att själva förändras. De finns i en enorm variation eftersom vart och ett oftast bara är inblandat i en reaktion. Enzymerna behöver bara finnas i ytterst små mängder för att påverka hastigheten för en reaktion.



I denna avhandling elektrokemiska biosensorer använts och i avhandlingen ingår studier av direkt och medierad elektronöverföring mellan immobiliserade enzymer (peroxidaser) och elektrodensyta, och studier om NADH oxidering med hjälp av mediator modifierade elektroder. Dessutom har biosensorer baserade på peroxidaser utvecklats för detektion av fenoliska ämnen i miljöprover. (Less)
Abstract
This thesis addresses: (1) studies of direct and mediated electron transfer and kinetics of peroxidases on electrodes and the development of amperometric peroxidase-based biosenors, (2) electrochemical and kinetic studies of NADH oxidation at mediator modified electrodes.



The direct and mediated electron transfer of horseradish and tobacco peroxidase were investigated on graphite electrodes by using wall-jet electrode methodology.



Biosensors based on novel plant peroxidases for determination of phenolic compounds were developed.



The electrochemical properties of different redox mediators immobilised on titanium phosphate, zirconium phosphate or silica gel were studied by means of... (More)
This thesis addresses: (1) studies of direct and mediated electron transfer and kinetics of peroxidases on electrodes and the development of amperometric peroxidase-based biosenors, (2) electrochemical and kinetic studies of NADH oxidation at mediator modified electrodes.



The direct and mediated electron transfer of horseradish and tobacco peroxidase were investigated on graphite electrodes by using wall-jet electrode methodology.



Biosensors based on novel plant peroxidases for determination of phenolic compounds were developed.



The electrochemical properties of different redox mediators immobilised on titanium phosphate, zirconium phosphate or silica gel were studied by means of cyclic voltammetry. The mediator properties for electrocatalytic oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) at the chemically modified electrodes are presented. For the kinetic investigations the rotating disk electrode methodology was used.



The interaction between some of the mediators and titanium phosphate was investigated by means of attenuated reflectance infrared spectroscopy and the possible leakage of the mediators from the electrode was investigated by using fast scan cyclic voltammetry and scanning electrochemical microscopy. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof. McNeil, Calum, University of Newcastle Upon Tyne, UK
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Electrochemistry, Elektrokemi, kinetic studies, silica gel, titanium phoshate, zirconium phosphate, NADH, redox mediator, phenolic compounds, hydrogen peroxide, electron transfer, graphite and carbon paste electrodes, peroxidase, biosensor
pages
208 pages
publisher
Analytical Chemistry, Lund University
defense location
Kemicentrum, Sölvegatan 39, Lund, hörsal B
defense date
2002-09-06 10:15
ISBN
91-7422-005-5
language
English
LU publication?
yes
id
46acff16-8811-45f6-b9a1-ea14f620d98b (old id 464787)
date added to LUP
2007-10-14 15:43:00
date last changed
2016-09-19 08:45:01
@phdthesis{46acff16-8811-45f6-b9a1-ea14f620d98b,
  abstract     = {This thesis addresses: (1) studies of direct and mediated electron transfer and kinetics of peroxidases on electrodes and the development of amperometric peroxidase-based biosenors, (2) electrochemical and kinetic studies of NADH oxidation at mediator modified electrodes.<br/><br>
<br/><br>
The direct and mediated electron transfer of horseradish and tobacco peroxidase were investigated on graphite electrodes by using wall-jet electrode methodology.<br/><br>
<br/><br>
Biosensors based on novel plant peroxidases for determination of phenolic compounds were developed.<br/><br>
<br/><br>
The electrochemical properties of different redox mediators immobilised on titanium phosphate, zirconium phosphate or silica gel were studied by means of cyclic voltammetry. The mediator properties for electrocatalytic oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) at the chemically modified electrodes are presented. For the kinetic investigations the rotating disk electrode methodology was used.<br/><br>
<br/><br>
The interaction between some of the mediators and titanium phosphate was investigated by means of attenuated reflectance infrared spectroscopy and the possible leakage of the mediators from the electrode was investigated by using fast scan cyclic voltammetry and scanning electrochemical microscopy.},
  author       = {Munteanu, Florentina},
  isbn         = {91-7422-005-5},
  keyword      = {Electrochemistry,Elektrokemi,kinetic studies,silica gel,titanium phoshate,zirconium phosphate,NADH,redox mediator,phenolic compounds,hydrogen peroxide,electron transfer,graphite and carbon paste electrodes,peroxidase,biosensor},
  language     = {eng},
  pages        = {208},
  publisher    = {Analytical Chemistry, Lund University},
  school       = {Lund University},
  title        = {Studies of Electrocatalytic Reactions of NADH at Mediator Modified Electrodes and of H2O2 at Peroxidase Modified Electrodes},
  year         = {2002},
}