Advanced

Design, Development and Applications of Highly Sensitive Protein-Based Capacitive Biosensors

Bontidean, Ibolya LU (2002)
Abstract
Highly sensitive biosensors were developed for environmental and medical applications. The biosensors were based on bioengineered proteins as biorecognition elements coupled to a highly sensitive capacitive transducer.



Heavy metal binding proteins like the metallothionein SmtA, regulatory protein MerR, periplasmic protein MerP and the synthetic phytochelatin EC20 were used for designing, constructing, and characterising biosensors for the determination of various heavy metal ions, i.e. mercury, copper, cadmium, zinc and lead, in a wide concentration range (fM-mM). The developed heavy metal biosensors had a useful storage stability (about two weeks) and could be regenerated using EDTA. The developed and optimised... (More)
Highly sensitive biosensors were developed for environmental and medical applications. The biosensors were based on bioengineered proteins as biorecognition elements coupled to a highly sensitive capacitive transducer.



Heavy metal binding proteins like the metallothionein SmtA, regulatory protein MerR, periplasmic protein MerP and the synthetic phytochelatin EC20 were used for designing, constructing, and characterising biosensors for the determination of various heavy metal ions, i.e. mercury, copper, cadmium, zinc and lead, in a wide concentration range (fM-mM). The developed heavy metal biosensors had a useful storage stability (about two weeks) and could be regenerated using EDTA. The developed and optimised biosensors were assessed in two practical applications, namely monitoring mercury ions in contaminated soil samples, and measuring inducer molecules.



The SmtA based biosensor was successfully used to monitor heavy metals in soil samples originating from a contaminated agricultural site in Denmark. The obtained results were compared to those given by a bacterial biosensor, a plant sensor, and the total amount of mercury contained in the soil. Both the protein-based and the bacterial biosensor, proved to accurately monitor the ion content, giving results well in agreement with those shown by the total amount.



By using the same sensing principle, biosensors based on lac repressor protein were developed for monitoring of different inducer molecules, e.g. IPTG, ONPG, and lactose, or DNA. The biosensor could differentiate between lac operator DNA contained in linearized plasmid DNA and circular plasmid DNA, and genomic DNA. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof., Belkin, Shimshon, The Fredy and Nadine Hermann Graduate School of Applied Science, The Hebrew University of Jerusalem, 91904-Jerusalem, Israel
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Bioteknik, Biotechnology, Contaminated soil, Heavy metals, Lac repressor, Phytochelatin, MerP, MerR, SmtA, Self-assembled monolayer, Biosensor, Capacitance
pages
174 pages
publisher
Ibolya Bontidean, Department of Biotechnology, Lund University
defense location
Center for Chemistry and Chemical Engineering, Lecture Hall C, Getingevägen 60, 221 00 Lund
defense date
2002-09-13 10:15
external identifiers
  • Other:ISRN: LUTKDH/TKBT—02/1062--SE
ISBN
91-89627-10-5
language
English
LU publication?
yes
id
975e4d22-d0a4-4ddf-908a-7bfd7ee9982c (old id 464837)
date added to LUP
2007-10-14 14:46:41
date last changed
2016-09-19 08:45:10
@phdthesis{975e4d22-d0a4-4ddf-908a-7bfd7ee9982c,
  abstract     = {Highly sensitive biosensors were developed for environmental and medical applications. The biosensors were based on bioengineered proteins as biorecognition elements coupled to a highly sensitive capacitive transducer.<br/><br>
<br/><br>
Heavy metal binding proteins like the metallothionein SmtA, regulatory protein MerR, periplasmic protein MerP and the synthetic phytochelatin EC20 were used for designing, constructing, and characterising biosensors for the determination of various heavy metal ions, i.e. mercury, copper, cadmium, zinc and lead, in a wide concentration range (fM-mM). The developed heavy metal biosensors had a useful storage stability (about two weeks) and could be regenerated using EDTA. The developed and optimised biosensors were assessed in two practical applications, namely monitoring mercury ions in contaminated soil samples, and measuring inducer molecules.<br/><br>
<br/><br>
The SmtA based biosensor was successfully used to monitor heavy metals in soil samples originating from a contaminated agricultural site in Denmark. The obtained results were compared to those given by a bacterial biosensor, a plant sensor, and the total amount of mercury contained in the soil. Both the protein-based and the bacterial biosensor, proved to accurately monitor the ion content, giving results well in agreement with those shown by the total amount.<br/><br>
<br/><br>
By using the same sensing principle, biosensors based on lac repressor protein were developed for monitoring of different inducer molecules, e.g. IPTG, ONPG, and lactose, or DNA. The biosensor could differentiate between lac operator DNA contained in linearized plasmid DNA and circular plasmid DNA, and genomic DNA.},
  author       = {Bontidean, Ibolya},
  isbn         = {91-89627-10-5},
  keyword      = {Bioteknik,Biotechnology,Contaminated soil,Heavy metals,Lac repressor,Phytochelatin,MerP,MerR,SmtA,Self-assembled monolayer,Biosensor,Capacitance},
  language     = {eng},
  pages        = {174},
  publisher    = {Ibolya Bontidean, Department of Biotechnology, Lund University},
  school       = {Lund University},
  title        = {Design, Development and Applications of Highly Sensitive Protein-Based Capacitive Biosensors},
  year         = {2002},
}