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Periostin Secreted by Mesenchymal Stem Cells Supports Tendon Formation in an Ectopic Mouse Model

Noack, Sandra ; Seiffart, Virginia ; Willbold, Elmar ; Laggies, Sandra ; Winkel, Andreas ; Shahab-Osterloh, Sandra ; Floerkemeier, Thilo ; Hertwig, Falk LU ; Steinhoff, Christine and Nuber, Ulrike LU , et al. (2014) In Stem Cells and Development 23(16). p.1844-1857
Abstract
True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T1/2 overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L + MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert... (More)
True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T1/2 overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L + MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert a major impact on tendon development. Gene expression analyses revealed four genes encoding secreted factors that are notably upregulated: periostin, C-type lectin domain family 3 (member b), RNase A4, and follistatin-like 1. These factors have not previously been implicated in tendon biology. Among these, periostin showed a specific expression in tenocytes of adult mouse Achilles tendon and in chondrocytes within the nonmineralized fibrocartilage zone of the enthesis with the calcaneus. Overexpression of periostin alone or in combination with constitutively active BMP receptor type in human mesenchymal stem cells and subsequent implantation into ectopic sites in mice demonstrated a reproducible moderate tenogenic capacity that has not been described before. Therefore, periostin may belong to the factors contributing to the development of tenogenic tissue. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cells and Development
volume
23
issue
16
pages
1844 - 1857
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000340523200002
  • scopus:84905637942
ISSN
1557-8534
DOI
10.1089/scd.2014.0124
language
English
LU publication?
yes
id
856dd2cb-b811-445d-aa45-ac8569349050 (old id 4656292)
date added to LUP
2016-04-01 11:00:33
date last changed
2025-04-04 15:15:02
@article{856dd2cb-b811-445d-aa45-ac8569349050,
  abstract     = {{True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T1/2 overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L + MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert a major impact on tendon development. Gene expression analyses revealed four genes encoding secreted factors that are notably upregulated: periostin, C-type lectin domain family 3 (member b), RNase A4, and follistatin-like 1. These factors have not previously been implicated in tendon biology. Among these, periostin showed a specific expression in tenocytes of adult mouse Achilles tendon and in chondrocytes within the nonmineralized fibrocartilage zone of the enthesis with the calcaneus. Overexpression of periostin alone or in combination with constitutively active BMP receptor type in human mesenchymal stem cells and subsequent implantation into ectopic sites in mice demonstrated a reproducible moderate tenogenic capacity that has not been described before. Therefore, periostin may belong to the factors contributing to the development of tenogenic tissue.}},
  author       = {{Noack, Sandra and Seiffart, Virginia and Willbold, Elmar and Laggies, Sandra and Winkel, Andreas and Shahab-Osterloh, Sandra and Floerkemeier, Thilo and Hertwig, Falk and Steinhoff, Christine and Nuber, Ulrike and Gross, Gerhard and Hoffmann, Andrea}},
  issn         = {{1557-8534}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{1844--1857}},
  publisher    = {{Mary Ann Liebert, Inc.}},
  series       = {{Stem Cells and Development}},
  title        = {{Periostin Secreted by Mesenchymal Stem Cells Supports Tendon Formation in an Ectopic Mouse Model}},
  url          = {{http://dx.doi.org/10.1089/scd.2014.0124}},
  doi          = {{10.1089/scd.2014.0124}},
  volume       = {{23}},
  year         = {{2014}},
}