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An Alkaline Active Cyclodextrin Glycosyltransferase from an Alkaliphilic Bacillus agaradhaerens

Martins, Rita LU (2003)
Abstract
Soda lakes represent the most stable naturally-occurring alkaline environments on Earth and harbour a diverse population of alkaliphiles. Alkaliphilic microorganisms have attracted a great interest due to their ability to produce extracellular enzymes that are active and stable at high pH values. Screening for alkaliphilic starch-hydrolyzing bacteria from samples of soda lakes at the Ethiopian Rift Valley (Lake Shalla, Lake Abijata, and Lake Aranguadi) resulted in the isolation of strains from the alkaliphilic species Bacillus pseudofirmus, B. cohnii, B. vedderi, and B. agaradhaerens, and one isolate closely related to the halophilic species Nesterenkonia halobia. A cyclodextrin glycosyltransferase (CGTase) from one of the B. agaradhaerens... (More)
Soda lakes represent the most stable naturally-occurring alkaline environments on Earth and harbour a diverse population of alkaliphiles. Alkaliphilic microorganisms have attracted a great interest due to their ability to produce extracellular enzymes that are active and stable at high pH values. Screening for alkaliphilic starch-hydrolyzing bacteria from samples of soda lakes at the Ethiopian Rift Valley (Lake Shalla, Lake Abijata, and Lake Aranguadi) resulted in the isolation of strains from the alkaliphilic species Bacillus pseudofirmus, B. cohnii, B. vedderi, and B. agaradhaerens, and one isolate closely related to the halophilic species Nesterenkonia halobia. A cyclodextrin glycosyltransferase (CGTase) from one of the B. agaradhaerens isolates (strain LS-3C) was purified and characterized with respect to catalysis, cyclodextrins (CDs) production, and stability. CDs are cyclic sugar molecules with a vast application in pharmaceutical, cosmetic and food industries, as well as in bioconversions and separation processes. The enzyme is a monomer with molecular weight of about 88 kDa and optimally active at pH 9.0 and 55 C. Although not so thermostable (only stable up to 40 C), this CGTase showed to have the widest pH span of stability (5.0-11.4) among other CGTases. Using maltodextrin as substrate, B. agaradhaerens LS-3C CGTase produced 89 % of beta-CD with only alpha-CD as secondary cyclic product. Product specificity could be further improved by addition of up to 14 mM CaCl2. Interestingly, compared to other CGTases, B. agaradhaerens LS-3C enzyme displayed significant hydrolysis activity in relation to its cyclization activity. CD ring-opening activity was minor, and coupling and disproportionation activities were also significantly lower than those of other reported CGTases. The gene encoding the enzyme was cloned and sequenced in order to investigate such differential characteristics at the molecular level. The deduced primary sequence of B. agaradhaerens CGTase notably revealed the presence of some residue replacements at the usually conserved positions in the sequence of these enzymes. The relation between such unusual replacements and the hydrolytic activity features of B. agaradhaerens LS-3C CGTase was analyzed. Few of the important amino acid replacements are indeed similar to those in the maltooligosaccharide-producing amylases, the so-called “intermediary” enzymes, which provides incipient evidence that B. agaradhaerens CGTase form a link between CGTases and alpha-amylases. Finally, B. agaradhaerens LS-3C cells were entrapped into Poly(vinyl alcohol) cryogel (PVA cryogel) beads and used as a source of immobilized CGTase. The immobilized biocatalyst was characterized and its application in a system with continuous CD removal by integrated adsorption was tested for beta-CD production. (Less)
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author
opponent
  • Dr Larsen, Kim, Department of Life Science, University of Aalborg, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark
organization
publishing date
type
Thesis
publication status
published
subject
keywords
cloning, characterization, purification, 16S rRNA gene analysis, screening, alkaliphiles, soda lakes, Bacillus agaradhaerens, cyclodextrins, cyclodextrin glycosyltransferase, alpha-amylase family, sequencing, immobilization, Biotechnology, Bioteknik
pages
51 pages
publisher
Rita Martins (rita.martins@biotek.lu.se) or Dept. of Biotechnology, Chemical Center, Lund University
defense location
Lecture Hall B Center of Chemistry and Chemical Engineering, Sölvegatan 39, Lund Institute of Technology
defense date
2003-06-13 10:30
external identifiers
  • other:ISRN: LUTKDH/TKBT--03/1069--SE
ISBN
91-89627-16-4
language
English
LU publication?
yes
id
6681757d-62df-4b5b-8181-d6854f433f8a (old id 465956)
date added to LUP
2007-10-14 14:16:32
date last changed
2016-09-19 08:45:10
@phdthesis{6681757d-62df-4b5b-8181-d6854f433f8a,
  abstract     = {Soda lakes represent the most stable naturally-occurring alkaline environments on Earth and harbour a diverse population of alkaliphiles. Alkaliphilic microorganisms have attracted a great interest due to their ability to produce extracellular enzymes that are active and stable at high pH values. Screening for alkaliphilic starch-hydrolyzing bacteria from samples of soda lakes at the Ethiopian Rift Valley (Lake Shalla, Lake Abijata, and Lake Aranguadi) resulted in the isolation of strains from the alkaliphilic species Bacillus pseudofirmus, B. cohnii, B. vedderi, and B. agaradhaerens, and one isolate closely related to the halophilic species Nesterenkonia halobia. A cyclodextrin glycosyltransferase (CGTase) from one of the B. agaradhaerens isolates (strain LS-3C) was purified and characterized with respect to catalysis, cyclodextrins (CDs) production, and stability. CDs are cyclic sugar molecules with a vast application in pharmaceutical, cosmetic and food industries, as well as in bioconversions and separation processes. The enzyme is a monomer with molecular weight of about 88 kDa and optimally active at pH 9.0 and 55 C. Although not so thermostable (only stable up to 40 C), this CGTase showed to have the widest pH span of stability (5.0-11.4) among other CGTases. Using maltodextrin as substrate, B. agaradhaerens LS-3C CGTase produced 89 % of beta-CD with only alpha-CD as secondary cyclic product. Product specificity could be further improved by addition of up to 14 mM CaCl2. Interestingly, compared to other CGTases, B. agaradhaerens LS-3C enzyme displayed significant hydrolysis activity in relation to its cyclization activity. CD ring-opening activity was minor, and coupling and disproportionation activities were also significantly lower than those of other reported CGTases. The gene encoding the enzyme was cloned and sequenced in order to investigate such differential characteristics at the molecular level. The deduced primary sequence of B. agaradhaerens CGTase notably revealed the presence of some residue replacements at the usually conserved positions in the sequence of these enzymes. The relation between such unusual replacements and the hydrolytic activity features of B. agaradhaerens LS-3C CGTase was analyzed. Few of the important amino acid replacements are indeed similar to those in the maltooligosaccharide-producing amylases, the so-called “intermediary” enzymes, which provides incipient evidence that B. agaradhaerens CGTase form a link between CGTases and alpha-amylases. Finally, B. agaradhaerens LS-3C cells were entrapped into Poly(vinyl alcohol) cryogel (PVA cryogel) beads and used as a source of immobilized CGTase. The immobilized biocatalyst was characterized and its application in a system with continuous CD removal by integrated adsorption was tested for beta-CD production.},
  author       = {Martins, Rita},
  isbn         = {91-89627-16-4},
  keyword      = {cloning,characterization,purification,16S rRNA gene analysis,screening,alkaliphiles,soda lakes,Bacillus agaradhaerens,cyclodextrins,cyclodextrin glycosyltransferase,alpha-amylase family,sequencing,immobilization,Biotechnology,Bioteknik},
  language     = {eng},
  pages        = {51},
  publisher    = {Rita Martins (rita.martins@biotek.lu.se) or Dept. of Biotechnology, Chemical Center, Lund University},
  school       = {Lund University},
  title        = {An Alkaline Active Cyclodextrin Glycosyltransferase from an Alkaliphilic Bacillus agaradhaerens},
  year         = {2003},
}