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The marine Gram-negative bacterium Novosphingobium sp. PP1Y as a potential source of novel metabolites with antioxidant activity

Petruk, Ganna LU orcid ; Roxo, Mariana ; De Lise, Federica ; Mensitieri, Francesca ; Notomista, Eugenio ; Wink, Michael ; Izzo, Viviana and Monti, Daria Maria (2019) In Biotechnology Letters 41(2). p.273-281
Abstract

Objective: The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated. Results: The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation. In the case of the cells, pre-treatment with methanolic extract was also able to maintain unaltered intracellular GSH levels and phosphorylation levels of mitogen-activated protein kinases p38. Instead, in the case of the worms,... (More)

Objective: The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated. Results: The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation. In the case of the cells, pre-treatment with methanolic extract was also able to maintain unaltered intracellular GSH levels and phosphorylation levels of mitogen-activated protein kinases p38. Instead, in the case of the worms, the extract was able to modulate the expression levels of stress response genes, by activating the transcription factor skn-1. Conclusions: From a biotechnological and economical point of view, antioxidants from microorganisms are convenient as they provide a valid alternative to chemical synthesis and respond to the ever-growing market demand for natural antioxidants.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Antioxidants, Carotenoids, Cellular stress, Gram-negative bacterium, Novosphingobium, Sphingomonadales
in
Biotechnology Letters
volume
41
issue
2
pages
273 - 281
publisher
Springer Science and Business Media B.V.
external identifiers
  • scopus:85058319517
  • pmid:30542947
ISSN
0141-5492
DOI
10.1007/s10529-018-02636-4
language
English
LU publication?
no
additional info
Publisher Copyright: © 2018, Springer Media B.V.
id
4674a9bd-dde4-4b72-92d7-20bbf00bb337
date added to LUP
2025-01-21 14:46:40
date last changed
2025-05-28 00:47:10
@article{4674a9bd-dde4-4b72-92d7-20bbf00bb337,
  abstract     = {{<p>Objective: The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated. Results: The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation. In the case of the cells, pre-treatment with methanolic extract was also able to maintain unaltered intracellular GSH levels and phosphorylation levels of mitogen-activated protein kinases p38. Instead, in the case of the worms, the extract was able to modulate the expression levels of stress response genes, by activating the transcription factor skn-1. Conclusions: From a biotechnological and economical point of view, antioxidants from microorganisms are convenient as they provide a valid alternative to chemical synthesis and respond to the ever-growing market demand for natural antioxidants.</p>}},
  author       = {{Petruk, Ganna and Roxo, Mariana and De Lise, Federica and Mensitieri, Francesca and Notomista, Eugenio and Wink, Michael and Izzo, Viviana and Monti, Daria Maria}},
  issn         = {{0141-5492}},
  keywords     = {{Antioxidants; Carotenoids; Cellular stress; Gram-negative bacterium; Novosphingobium; Sphingomonadales}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{273--281}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{The marine Gram-negative bacterium Novosphingobium sp. PP1Y as a potential source of novel metabolites with antioxidant activity}},
  url          = {{http://dx.doi.org/10.1007/s10529-018-02636-4}},
  doi          = {{10.1007/s10529-018-02636-4}},
  volume       = {{41}},
  year         = {{2019}},
}