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Abundance of Broad Bacterial Taxa in the Sargasso Sea Explained by Environmental Conditions but Not Water Mass

Sjöstedt, Johanna LU ; Martiny, J. B. H.; Munk, P. and Riemannd, L. (2014) In Applied and Environmental Microbiology 80(9). p.2786-2795
Abstract
To explore the potential linkage between distribution of marine bacterioplankton groups, environmental conditions, and water mass, we investigated the factors determining the abundance of bacterial taxa across the hydrographically complex Subtropical Convergence Zone in the Sargasso Sea. Based on information from 16S rRNA gene clone libraries from various locations and two depths, abundances of the predominant taxa (eubacteria, Archaea, Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and the Roseobacter, SAR11, and SAR86 clades) were quantified by real-time PCR. In addition, the abundances of Synechococcus, Prochlorococcus, and picoalgae were determined by flow cytometry. Linear multiple-regression models determining the relative... (More)
To explore the potential linkage between distribution of marine bacterioplankton groups, environmental conditions, and water mass, we investigated the factors determining the abundance of bacterial taxa across the hydrographically complex Subtropical Convergence Zone in the Sargasso Sea. Based on information from 16S rRNA gene clone libraries from various locations and two depths, abundances of the predominant taxa (eubacteria, Archaea, Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and the Roseobacter, SAR11, and SAR86 clades) were quantified by real-time PCR. In addition, the abundances of Synechococcus, Prochlorococcus, and picoalgae were determined by flow cytometry. Linear multiple-regression models determining the relative effects of eight environmental variables and of water mass explained 35 to 86% of the variation in abundance of the quantified taxa, even though only one to three variables were significantly related to any particular taxon's abundance. Most of the variation in abundance was explained by depth and chlorophyll a. The predominant phototrophs, Prochlorococcus and picoalgae, were negatively correlated with phosphate, whereas eubacteria, heterotrophic bacteria, and SAR86 were negatively correlated with nitrite. Water mass showed limited importance for explaining the abundance of the taxonomical groups (significant only for Roseobacter, explaining 14% of the variation). The results suggest the potential for predicting the abundance of broad bacterioplankton groups throughout the Sargasso Sea using only a few environmental parameters. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
16s ribosomal-rna, gel-electrophoresis analysis, polymerase-chain-reaction, dissolved organic-matter, atlantic-ocean, microbial communities, pacific-ocean, spatial-patterns, quantitative, pcr, marine-bacteria
in
Applied and Environmental Microbiology
volume
80
issue
9
pages
2786 - 2795
publisher
American Society for Microbiology
external identifiers
  • scopus:84898605116
ISSN
0099-2240
DOI
10.1128/aem.00099-14
language
English
LU publication?
no
id
cd70c3c1-0633-4000-a474-0278b9cd84dc (old id 4690188)
date added to LUP
2014-09-29 15:57:38
date last changed
2017-11-12 03:02:56
@article{cd70c3c1-0633-4000-a474-0278b9cd84dc,
  abstract     = {To explore the potential linkage between distribution of marine bacterioplankton groups, environmental conditions, and water mass, we investigated the factors determining the abundance of bacterial taxa across the hydrographically complex Subtropical Convergence Zone in the Sargasso Sea. Based on information from 16S rRNA gene clone libraries from various locations and two depths, abundances of the predominant taxa (eubacteria, Archaea, Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and the Roseobacter, SAR11, and SAR86 clades) were quantified by real-time PCR. In addition, the abundances of Synechococcus, Prochlorococcus, and picoalgae were determined by flow cytometry. Linear multiple-regression models determining the relative effects of eight environmental variables and of water mass explained 35 to 86% of the variation in abundance of the quantified taxa, even though only one to three variables were significantly related to any particular taxon's abundance. Most of the variation in abundance was explained by depth and chlorophyll a. The predominant phototrophs, Prochlorococcus and picoalgae, were negatively correlated with phosphate, whereas eubacteria, heterotrophic bacteria, and SAR86 were negatively correlated with nitrite. Water mass showed limited importance for explaining the abundance of the taxonomical groups (significant only for Roseobacter, explaining 14% of the variation). The results suggest the potential for predicting the abundance of broad bacterioplankton groups throughout the Sargasso Sea using only a few environmental parameters.},
  author       = {Sjöstedt, Johanna and Martiny, J. B. H. and Munk, P. and Riemannd, L.},
  issn         = {0099-2240},
  keyword      = {16s ribosomal-rna,gel-electrophoresis analysis,polymerase-chain-reaction,dissolved organic-matter,atlantic-ocean,microbial communities,pacific-ocean,spatial-patterns,quantitative,pcr,marine-bacteria},
  language     = {eng},
  number       = {9},
  pages        = {2786--2795},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Abundance of Broad Bacterial Taxa in the Sargasso Sea Explained by Environmental Conditions but Not Water Mass},
  url          = {http://dx.doi.org/10.1128/aem.00099-14},
  volume       = {80},
  year         = {2014},
}