Advanced

BDNF increases survival and neuronal differentiation of human neural precursor cells cotransplanted with a nanofiber gel to the auditory nerve in a rat model of neuronal damage.

Jiao, Yu; Palmgren, Björn; Novozhilova, Ekaterina; Englund Johansson, Ulrica LU ; Spieles-Engemann, Anne L; Kale, Ajay; Stupp, Samuel I and Olivius, Petri (2014) In BioMed Research International 2014.
Abstract
Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of β-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the... (More)
Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of β-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus. Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BioMed Research International
volume
2014
publisher
Hindawi Publishing Corporation
external identifiers
  • wos:000347810500001
  • pmid:25243135
  • scopus:84935920929
ISSN
2314-6133
DOI
10.1155/2014/356415
language
English
LU publication?
yes
id
afb1514f-ff3d-4359-bd72-ff6c8b92a379 (old id 4691082)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25243135?dopt=Abstract
date added to LUP
2014-10-08 15:22:05
date last changed
2017-07-23 03:27:04
@article{afb1514f-ff3d-4359-bd72-ff6c8b92a379,
  abstract     = {Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of β-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus. Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN).},
  articleno    = {356415},
  author       = {Jiao, Yu and Palmgren, Björn and Novozhilova, Ekaterina and Englund Johansson, Ulrica and Spieles-Engemann, Anne L and Kale, Ajay and Stupp, Samuel I and Olivius, Petri},
  issn         = {2314-6133},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {BioMed Research International},
  title        = {BDNF increases survival and neuronal differentiation of human neural precursor cells cotransplanted with a nanofiber gel to the auditory nerve in a rat model of neuronal damage.},
  url          = {http://dx.doi.org/10.1155/2014/356415},
  volume       = {2014},
  year         = {2014},
}