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A capacitive DNA sensor-based test for simple and sensitive analysis of antibiotic resistance in field setting.

Liu, Yanling; Hedström, Martin; Chen, Dongfeng LU ; Fan, Xiaolong LU and Mattiasson, Bo (2015) In Biosensors & Bioelectronics 64. p.255-259
Abstract
To meet urgent needs for solving serious worldwide drug-resistance problems, a sensitive label-free capacitive sensor developed in our group was investigated as a tool to be applied in the field of antibiotic resistance genotyping, for instance the detection of ampicillin resistance gene (ampR). Proof-of-concept data demonstrated its detection sensitivity of pico-molar without any signal amplification step and a dynamic range of at least three orders of magnitude. The detection limits of less than 1pM for the single-stranded ampR oligonucleotide and 4pM for the double-stranded target can reliably be achieved after only 2.5min sample reaction. Reusability of the probe-functionalized disposable electrode was investigated by comparing... (More)
To meet urgent needs for solving serious worldwide drug-resistance problems, a sensitive label-free capacitive sensor developed in our group was investigated as a tool to be applied in the field of antibiotic resistance genotyping, for instance the detection of ampicillin resistance gene (ampR). Proof-of-concept data demonstrated its detection sensitivity of pico-molar without any signal amplification step and a dynamic range of at least three orders of magnitude. The detection limits of less than 1pM for the single-stranded ampR oligonucleotide and 4pM for the double-stranded target can reliably be achieved after only 2.5min sample reaction. Reusability of the probe-functionalized disposable electrode was investigated by comparing different regeneration solutions; mix of 25mM NaOH/30% formamide was employed to regenerate the electrode for at least six cycles without significant loss of sensing ability. Assay is performed automatically and result is retrieved in 20min. The developed sensitive genotyping tool is expected to provide simple, fast and affordable screening for monitoring spread of antibiotic resistances, which is suitable for testing in field setting. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
64
pages
255 - 259
publisher
Elsevier
external identifiers
  • pmid:25238540
  • wos:000345059100038
  • scopus:84907167841
ISSN
1873-4235
DOI
10.1016/j.bios.2014.08.084
language
English
LU publication?
yes
id
a2d5c23f-eae1-40d9-8669-13bfe8349f97 (old id 4691219)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25238540?dopt=Abstract
date added to LUP
2014-10-07 22:20:25
date last changed
2017-01-01 03:22:35
@article{a2d5c23f-eae1-40d9-8669-13bfe8349f97,
  abstract     = {To meet urgent needs for solving serious worldwide drug-resistance problems, a sensitive label-free capacitive sensor developed in our group was investigated as a tool to be applied in the field of antibiotic resistance genotyping, for instance the detection of ampicillin resistance gene (ampR). Proof-of-concept data demonstrated its detection sensitivity of pico-molar without any signal amplification step and a dynamic range of at least three orders of magnitude. The detection limits of less than 1pM for the single-stranded ampR oligonucleotide and 4pM for the double-stranded target can reliably be achieved after only 2.5min sample reaction. Reusability of the probe-functionalized disposable electrode was investigated by comparing different regeneration solutions; mix of 25mM NaOH/30% formamide was employed to regenerate the electrode for at least six cycles without significant loss of sensing ability. Assay is performed automatically and result is retrieved in 20min. The developed sensitive genotyping tool is expected to provide simple, fast and affordable screening for monitoring spread of antibiotic resistances, which is suitable for testing in field setting.},
  author       = {Liu, Yanling and Hedström, Martin and Chen, Dongfeng and Fan, Xiaolong and Mattiasson, Bo},
  issn         = {1873-4235},
  language     = {eng},
  pages        = {255--259},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {A capacitive DNA sensor-based test for simple and sensitive analysis of antibiotic resistance in field setting.},
  url          = {http://dx.doi.org/10.1016/j.bios.2014.08.084},
  volume       = {64},
  year         = {2015},
}