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Solid-to-fluid DNA transition inside HSV-1 capsid close to the temperature of infection.

Sae-Ueng, Udom; Li, Dong; Zuo, Xiaobing; Huffman, Jamie B; Homa, Fred L; Rau, Donald and Evilevitch, Alex LU (2014) In Nature Chemical Biology 10(10). p.861-861
Abstract
DNA in the human Herpes simplex virus type 1 (HSV-1) capsid is packaged to a tight density. This leads to tens of atmospheres of internal pressure responsible for the delivery of the herpes genome into the cell nucleus. In this study we show that, despite its liquid crystalline state inside the capsid, the DNA is fluid-like, which facilitates its ejection into the cell nucleus during infection. We found that the sliding friction between closely packaged DNA strands, caused by interstrand repulsive interactions, is reduced by the ionic environment of epithelial cells and neurons susceptible to herpes infection. However, variations in the ionic conditions corresponding to neuronal activity can restrict DNA mobility in the capsid, making it... (More)
DNA in the human Herpes simplex virus type 1 (HSV-1) capsid is packaged to a tight density. This leads to tens of atmospheres of internal pressure responsible for the delivery of the herpes genome into the cell nucleus. In this study we show that, despite its liquid crystalline state inside the capsid, the DNA is fluid-like, which facilitates its ejection into the cell nucleus during infection. We found that the sliding friction between closely packaged DNA strands, caused by interstrand repulsive interactions, is reduced by the ionic environment of epithelial cells and neurons susceptible to herpes infection. However, variations in the ionic conditions corresponding to neuronal activity can restrict DNA mobility in the capsid, making it more solid-like. This can inhibit intranuclear DNA release and interfere with viral replication. In addition, the temperature of the human host (37 °C) induces a disordering transition of the encapsidated herpes genome, which reduces interstrand interactions and provides genome mobility required for infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Chemical Biology
volume
10
issue
10
pages
861 - 861
publisher
Nature Publishing Group
external identifiers
  • pmid:25195012
  • wos:000342462100013
  • scopus:84922061254
ISSN
1552-4469
DOI
10.1038/nchembio.1628
language
English
LU publication?
yes
id
d2e0fcf6-f1d9-4392-bc21-74d14e32261e (old id 4692145)
date added to LUP
2014-10-07 16:49:21
date last changed
2017-10-01 03:01:48
@article{d2e0fcf6-f1d9-4392-bc21-74d14e32261e,
  abstract     = {DNA in the human Herpes simplex virus type 1 (HSV-1) capsid is packaged to a tight density. This leads to tens of atmospheres of internal pressure responsible for the delivery of the herpes genome into the cell nucleus. In this study we show that, despite its liquid crystalline state inside the capsid, the DNA is fluid-like, which facilitates its ejection into the cell nucleus during infection. We found that the sliding friction between closely packaged DNA strands, caused by interstrand repulsive interactions, is reduced by the ionic environment of epithelial cells and neurons susceptible to herpes infection. However, variations in the ionic conditions corresponding to neuronal activity can restrict DNA mobility in the capsid, making it more solid-like. This can inhibit intranuclear DNA release and interfere with viral replication. In addition, the temperature of the human host (37 °C) induces a disordering transition of the encapsidated herpes genome, which reduces interstrand interactions and provides genome mobility required for infection.},
  author       = {Sae-Ueng, Udom and Li, Dong and Zuo, Xiaobing and Huffman, Jamie B and Homa, Fred L and Rau, Donald and Evilevitch, Alex},
  issn         = {1552-4469},
  language     = {eng},
  number       = {10},
  pages        = {861--861},
  publisher    = {Nature Publishing Group},
  series       = {Nature Chemical Biology},
  title        = {Solid-to-fluid DNA transition inside HSV-1 capsid close to the temperature of infection.},
  url          = {http://dx.doi.org/10.1038/nchembio.1628},
  volume       = {10},
  year         = {2014},
}