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Autophagy and ER-stress contribute to photoreceptor degeneration in cultured adult porcine retina.

Mohlin, Camilla; Taylor, Linnéa LU ; Ghosh, Fredrik LU and Johansson, Kjell LU (2014) In Brain Research 1585(Aug 28). p.167-183
Abstract
The aim of this study was to investigate rod and cone photoreceptor degeneration in organotypic cultures of adult porcine retina. Our hypothesis was that the photoreceptors accumulate opsins, which, together with exposure to cyclic dim light illumination, induce autophagy and endoplasmic reticulum stress (ER-stress) to overcome damaging protein overload. For this purpose, retinas were cultured for 48h and 72h during which they were illuminated with dim light for 8h/day; specimens were analyzed by means of immunohistochemistry, Western blot, real-time polymerase chain reaction (PCR) and transmission electron microscopy. ER-stress and photoreceptor degeneration was observed in conventionally cultured retinas. The additional stress in the... (More)
The aim of this study was to investigate rod and cone photoreceptor degeneration in organotypic cultures of adult porcine retina. Our hypothesis was that the photoreceptors accumulate opsins, which, together with exposure to cyclic dim light illumination, induce autophagy and endoplasmic reticulum stress (ER-stress) to overcome damaging protein overload. For this purpose, retinas were cultured for 48h and 72h during which they were illuminated with dim light for 8h/day; specimens were analyzed by means of immunohistochemistry, Western blot, real-time polymerase chain reaction (PCR) and transmission electron microscopy. ER-stress and photoreceptor degeneration was observed in conventionally cultured retinas. The additional stress in the form of dim light illumination for 8h/day resulted in increased levels of the ER-stress markers GRP78/BiP and CHOP, as well as increased level of active caspase-12. Increased autophagic processes in cone and rod photoreceptors were detected by LC3B-II increases and occurrence of autophagosomes at the ultrastructural level. Illumination also resulted in altered protein expression for autophagy inducers such as p62 and Beclin-1. Moreover, there was a decrease in phosphorylated mammalian target of rapamycin (mTOR), which further indicate an increase of autophagy. Rod and cone photoreceptors in retinas from a diurnal animal that were exposed to dim light illumination in vitro displayed autophagy and ER-stress processes. As no alteration of rhodopsin mRNA was observed, autophagy and ER-stress are suggested to decrease rhodopsin protein at the posttranscriptional level. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Brain Research
volume
1585
issue
Aug 28
pages
167 - 183
publisher
Elsevier
external identifiers
  • pmid:25173074
  • wos:000343840300017
  • scopus:84907653256
ISSN
1872-6240
DOI
10.1016/j.brainres.2014.08.055
language
English
LU publication?
yes
id
b7ed886b-1c29-4259-8792-451730751e4d (old id 4692866)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25173074?dopt=Abstract
date added to LUP
2014-10-01 10:32:46
date last changed
2017-05-21 03:25:10
@article{b7ed886b-1c29-4259-8792-451730751e4d,
  abstract     = {The aim of this study was to investigate rod and cone photoreceptor degeneration in organotypic cultures of adult porcine retina. Our hypothesis was that the photoreceptors accumulate opsins, which, together with exposure to cyclic dim light illumination, induce autophagy and endoplasmic reticulum stress (ER-stress) to overcome damaging protein overload. For this purpose, retinas were cultured for 48h and 72h during which they were illuminated with dim light for 8h/day; specimens were analyzed by means of immunohistochemistry, Western blot, real-time polymerase chain reaction (PCR) and transmission electron microscopy. ER-stress and photoreceptor degeneration was observed in conventionally cultured retinas. The additional stress in the form of dim light illumination for 8h/day resulted in increased levels of the ER-stress markers GRP78/BiP and CHOP, as well as increased level of active caspase-12. Increased autophagic processes in cone and rod photoreceptors were detected by LC3B-II increases and occurrence of autophagosomes at the ultrastructural level. Illumination also resulted in altered protein expression for autophagy inducers such as p62 and Beclin-1. Moreover, there was a decrease in phosphorylated mammalian target of rapamycin (mTOR), which further indicate an increase of autophagy. Rod and cone photoreceptors in retinas from a diurnal animal that were exposed to dim light illumination in vitro displayed autophagy and ER-stress processes. As no alteration of rhodopsin mRNA was observed, autophagy and ER-stress are suggested to decrease rhodopsin protein at the posttranscriptional level.},
  author       = {Mohlin, Camilla and Taylor, Linnéa and Ghosh, Fredrik and Johansson, Kjell},
  issn         = {1872-6240},
  language     = {eng},
  number       = {Aug 28},
  pages        = {167--183},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Autophagy and ER-stress contribute to photoreceptor degeneration in cultured adult porcine retina.},
  url          = {http://dx.doi.org/10.1016/j.brainres.2014.08.055},
  volume       = {1585},
  year         = {2014},
}