Targeting miRNA by CRISPR/Cas in cancer : advantages and challenges
(2023) In Military Medical Research 10(1).- Abstract
Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed biomedical research and provided entirely new models to analyze every aspect of biomedical sciences during the last decade. In the study of cancer, the CRISPR/CRISPR-associated protein (Cas) system opens new avenues into issues that were once unknown in our knowledge of the noncoding genome, tumor heterogeneity, and precision medicines. CRISPR/Cas-based gene-editing technology now allows for the precise and permanent targeting of mutations and provides an opportunity to target small non-coding RNAs such as microRNAs (miRNAs). However, the development of effective and safe cancer gene editing therapy is highly dependent on proper design to be innocuous to... (More)
Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed biomedical research and provided entirely new models to analyze every aspect of biomedical sciences during the last decade. In the study of cancer, the CRISPR/CRISPR-associated protein (Cas) system opens new avenues into issues that were once unknown in our knowledge of the noncoding genome, tumor heterogeneity, and precision medicines. CRISPR/Cas-based gene-editing technology now allows for the precise and permanent targeting of mutations and provides an opportunity to target small non-coding RNAs such as microRNAs (miRNAs). However, the development of effective and safe cancer gene editing therapy is highly dependent on proper design to be innocuous to normal cells and prevent introducing other abnormalities. This study aims to highlight the cutting-edge approaches in cancer-gene editing therapy based on the CRISPR/Cas technology to target miRNAs in cancer therapy. Furthermore, we highlight the potential challenges in CRISPR/Cas-mediated miRNA gene editing and offer advanced strategies to overcome them.
(Less)
- author
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cancer therapy, CRISPR, CRISPR/Cas12, CRISPR/Cas9, Gene editing, miRNAs
- in
- Military Medical Research
- volume
- 10
- issue
- 1
- article number
- 32
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:37460924
- scopus:85165021554
- ISSN
- 2095-7467
- DOI
- 10.1186/s40779-023-00468-6
- language
- English
- LU publication?
- yes
- id
- 4697bd5f-97b7-48ac-ab25-6552cd6a1936
- date added to LUP
- 2023-08-23 15:24:02
- date last changed
- 2024-04-20 01:23:49
@article{4697bd5f-97b7-48ac-ab25-6552cd6a1936, abstract = {{<p>Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed biomedical research and provided entirely new models to analyze every aspect of biomedical sciences during the last decade. In the study of cancer, the CRISPR/CRISPR-associated protein (Cas) system opens new avenues into issues that were once unknown in our knowledge of the noncoding genome, tumor heterogeneity, and precision medicines. CRISPR/Cas-based gene-editing technology now allows for the precise and permanent targeting of mutations and provides an opportunity to target small non-coding RNAs such as microRNAs (miRNAs). However, the development of effective and safe cancer gene editing therapy is highly dependent on proper design to be innocuous to normal cells and prevent introducing other abnormalities. This study aims to highlight the cutting-edge approaches in cancer-gene editing therapy based on the CRISPR/Cas technology to target miRNAs in cancer therapy. Furthermore, we highlight the potential challenges in CRISPR/Cas-mediated miRNA gene editing and offer advanced strategies to overcome them.</p>}}, author = {{Hussen, Bashdar Mahmud and Rasul, Mohammed Fatih and Abdullah, Snur Rasool and Hidayat, Hazha Jamal and Faraj, Goran Sedeeq Hama and Ali, Fattma Abodi and Salihi, Abbas and Baniahmad, Aria and Ghafouri-Fard, Soudeh and Rahman, Milladur and Glassy, Mark C. and Branicki, Wojciech and Taheri, Mohammad}}, issn = {{2095-7467}}, keywords = {{Cancer therapy; CRISPR; CRISPR/Cas12; CRISPR/Cas9; Gene editing; miRNAs}}, language = {{eng}}, number = {{1}}, publisher = {{BioMed Central (BMC)}}, series = {{Military Medical Research}}, title = {{Targeting miRNA by CRISPR/Cas in cancer : advantages and challenges}}, url = {{http://dx.doi.org/10.1186/s40779-023-00468-6}}, doi = {{10.1186/s40779-023-00468-6}}, volume = {{10}}, year = {{2023}}, }