Advanced

Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.

Aragao, Rosa LU ; Alvarez Aliaga, Teresa LU and Mattiasson, Bo LU (2013) In Biotechnology Letters 35(3). p.397-405
Abstract
Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix... (More)
Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Microcultivation, Single cell, Alginate, Anaerobic bacteria
in
Biotechnology Letters
volume
35
issue
3
pages
397 - 405
publisher
Springer
external identifiers
  • wos:000314788400014
  • pmid:23224821
  • scopus:84873999308
  • pmid:23224821
ISSN
1573-6776
DOI
10.1007/s10529-012-1094-1
language
English
LU publication?
yes
id
469910e7-2c22-43bf-bb8a-bbb46c6ea6ba (old id 3347417)
date added to LUP
2016-04-01 13:14:40
date last changed
2020-01-12 11:34:45
@article{469910e7-2c22-43bf-bb8a-bbb46c6ea6ba,
  abstract     = {Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose.},
  author       = {Aragao, Rosa and Alvarez Aliaga, Teresa and Mattiasson, Bo},
  issn         = {1573-6776},
  language     = {eng},
  number       = {3},
  pages        = {397--405},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.},
  url          = {http://dx.doi.org/10.1007/s10529-012-1094-1},
  doi          = {10.1007/s10529-012-1094-1},
  volume       = {35},
  year         = {2013},
}