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Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production.

Cavero Olguin, Victor Hugo LU ; Hatti-Kaul, Rajni LU ; Cardenas, Oscar Victor ; Valverde, Marcelo Gutierrez ; Romero, Danitza Xiomara and Alvarez-Aliaga, Maria Teresa (2019) In World Journal of Microbiology and Biotechnology 35(101).
Abstract
Propionibacterium acidipropionici produces propionic acid from different sugars and glycerol; the production can be improved by high cell density fermentations using immobilized cells that help to overcome the limitations of the non-productive lag phase and product inhibition. In this study, the use of stress factors to induce P. acidipropionici to form biofilm and its use as an immobilization procedure in fermentations in bioreactors for producing propionic acid was investigated. Citric acid and sodium chloride increased exopolysaccharide production, biofilm capacity index and trehalose production. Analysis of the expression of trehalose synthesis-related genes otsA and treY by RT-qPCR showed significantly increased expression of only... (More)
Propionibacterium acidipropionici produces propionic acid from different sugars and glycerol; the production can be improved by high cell density fermentations using immobilized cells that help to overcome the limitations of the non-productive lag phase and product inhibition. In this study, the use of stress factors to induce P. acidipropionici to form biofilm and its use as an immobilization procedure in fermentations in bioreactors for producing propionic acid was investigated. Citric acid and sodium chloride increased exopolysaccharide production, biofilm capacity index and trehalose production. Analysis of the expression of trehalose synthesis-related genes otsA and treY by RT-qPCR showed significantly increased expression of only treY during log phase with citric acid, while FISH analysis showed expression of treY and luxS under the influence of both stress factors. The stress factors were then used for development of microbial biofilms as immobilization procedure on Poraver® and AnoxKaldnes® carriers in recycle batch reactors for propionic acid production from 20 g/L glycerol. Highest productivities of 0.7 and 0.78 g/L/h were obtained in Poraver® reactors, and 0.39 and 0.43 g/L/h in AnoxKaldnes® reactors with citric acid and NaCl, respectively. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biofilm; immobilization; propionic acid; stress factor; trehalose
in
World Journal of Microbiology and Biotechnology
volume
35
issue
101
article number
101
pages
12 pages
publisher
Springer
external identifiers
  • scopus:85067862492
  • pmid:31236717
ISSN
0959-3993
DOI
10.1007/s11274-019-2679-9
language
English
LU publication?
yes
id
46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc
date added to LUP
2019-06-27 22:18:30
date last changed
2022-04-26 02:17:31
@article{46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc,
  abstract     = {{Propionibacterium acidipropionici produces propionic acid from different sugars and glycerol; the production can be improved by high cell density fermentations using immobilized cells that help to overcome the limitations of the non-productive lag phase and product inhibition. In this study, the use of stress factors to induce P. acidipropionici to form biofilm and its use as an immobilization procedure in fermentations in bioreactors for producing propionic acid was investigated. Citric acid and sodium chloride increased exopolysaccharide production, biofilm capacity index and trehalose production. Analysis of the expression of trehalose synthesis-related genes otsA and treY by RT-qPCR showed significantly increased expression of only treY during log phase with citric acid, while FISH analysis showed expression of treY and luxS under the influence of both stress factors. The stress factors were then used for development of microbial biofilms as immobilization procedure on Poraver® and AnoxKaldnes® carriers in recycle batch reactors for propionic acid production from 20 g/L glycerol. Highest productivities of 0.7 and 0.78 g/L/h were obtained in Poraver® reactors, and 0.39 and 0.43 g/L/h in AnoxKaldnes® reactors with citric acid and NaCl, respectively.}},
  author       = {{Cavero Olguin, Victor Hugo and Hatti-Kaul, Rajni and Cardenas, Oscar Victor and Valverde, Marcelo Gutierrez and Romero, Danitza Xiomara and Alvarez-Aliaga, Maria Teresa}},
  issn         = {{0959-3993}},
  keywords     = {{Biofilm; immobilization; propionic acid; stress factor; trehalose}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{101}},
  publisher    = {{Springer}},
  series       = {{World Journal of Microbiology and Biotechnology}},
  title        = {{Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production.}},
  url          = {{http://dx.doi.org/10.1007/s11274-019-2679-9}},
  doi          = {{10.1007/s11274-019-2679-9}},
  volume       = {{35}},
  year         = {{2019}},
}