Hfq-Assisted RsmA Regulation Is Central to Pseudomonas aeruginosa Biofilm Polysaccharide PEL Expression
Irie, Yasuhiko ; La Mensa, Agnese ; Murina, Victoriia ; Hauryliuk, Vasili LU
; Tenson, Tanel
and Shingler, Victoria
(2020)
In Frontiers in Microbiology
11.
p.1-15
- Abstract
To appropriately switch between sessile and motile lifestyles, bacteria control expression of biofilm-associated genes through multiple regulatory elements. In Pseudomonas aeruginosa, the post-transcriptional regulator RsmA has been implicated in the control of various genes including those related to biofilms, but much of the evidence for these links is limited to transcriptomic and phenotypic studies. RsmA binds to target mRNAs to modulate translation by affecting ribosomal access and/or mRNA stability. Here, we trace a global regulatory role of RsmA to inhibition of the expression of Vfr—a transcription factor that inhibits transcriptional regulator FleQ. FleQ directly controls biofilm-associated genes that encode the PEL... (More)
To appropriately switch between sessile and motile lifestyles, bacteria control expression of biofilm-associated genes through multiple regulatory elements. In Pseudomonas aeruginosa, the post-transcriptional regulator RsmA has been implicated in the control of various genes including those related to biofilms, but much of the evidence for these links is limited to transcriptomic and phenotypic studies. RsmA binds to target mRNAs to modulate translation by affecting ribosomal access and/or mRNA stability. Here, we trace a global regulatory role of RsmA to inhibition of the expression of Vfr—a transcription factor that inhibits transcriptional regulator FleQ. FleQ directly controls biofilm-associated genes that encode the PEL polysaccharide biosynthesis machinery. Furthermore, we show that RsmA alone cannot bind vfr mRNA but requires the assistance of RNA chaperone protein Hfq. This is the first example where a RsmA protein family member requires another protein for binding to its target RNA.
(Less)
- author
- Irie, Yasuhiko
; La Mensa, Agnese
; Murina, Victoriia
; Hauryliuk, Vasili
LU
; Tenson, Tanel
and Shingler, Victoria
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- keywords
- FleQ, Hfq, Pseudomonas aeruginosa, RsmA, Vfr
- in
- Frontiers in Microbiology
- volume
- 11
- article number
- 482585
- pages
- 1 - 15
- publisher
- Frontiers Media S. A.
- external identifiers
-
- pmid:33281751
- scopus:85097189906
- ISSN
- 1664-302X
- DOI
- 10.3389/fmicb.2020.482585
- language
- English
- LU publication?
- no
- additional info
- Funding Information: YI would like to thank Matt Parsek for his more than a decade of scientific and moral support that led to the culmination and conclusion of this project. The authors would like to thank Elisabeth Sonnleitner for her generous gifts of hfq deletion construct and Hfq over-expression plasmids. Funding. This work was supported by the J C Kempe and S M Kempe Foundation (JCK-1523 to VS), the Swedish Research Council (grant numbers 2016-02047 to VS and 2017-03783 to VH), and the University of Tartu Institute of Technology Grant (PLTTIIRIE to YI). Publisher Copyright: © Copyright © 2020 Irie, La Mensa, Murina, Hauryliuk, Tenson and Shingler. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
- id
- 46f04317-49b7-4307-a8ae-a910d71af750
- date added to LUP
- 2021-09-24 20:31:02
- date last changed
- 2024-04-06 09:23:33
@article{46f04317-49b7-4307-a8ae-a910d71af750,
abstract = {{<p>To appropriately switch between sessile and motile lifestyles, bacteria control expression of biofilm-associated genes through multiple regulatory elements. In Pseudomonas aeruginosa, the post-transcriptional regulator RsmA has been implicated in the control of various genes including those related to biofilms, but much of the evidence for these links is limited to transcriptomic and phenotypic studies. RsmA binds to target mRNAs to modulate translation by affecting ribosomal access and/or mRNA stability. Here, we trace a global regulatory role of RsmA to inhibition of the expression of Vfr—a transcription factor that inhibits transcriptional regulator FleQ. FleQ directly controls biofilm-associated genes that encode the PEL polysaccharide biosynthesis machinery. Furthermore, we show that RsmA alone cannot bind vfr mRNA but requires the assistance of RNA chaperone protein Hfq. This is the first example where a RsmA protein family member requires another protein for binding to its target RNA.</p>}},
author = {{Irie, Yasuhiko and La Mensa, Agnese and Murina, Victoriia and Hauryliuk, Vasili and Tenson, Tanel and Shingler, Victoria}},
issn = {{1664-302X}},
keywords = {{FleQ; Hfq; Pseudomonas aeruginosa; RsmA; Vfr}},
language = {{eng}},
pages = {{1--15}},
publisher = {{Frontiers Media S. A.}},
series = {{Frontiers in Microbiology}},
title = {{Hfq-Assisted RsmA Regulation Is Central to Pseudomonas aeruginosa Biofilm Polysaccharide PEL Expression}},
url = {{http://dx.doi.org/10.3389/fmicb.2020.482585}},
doi = {{10.3389/fmicb.2020.482585}},
volume = {{11}},
year = {{2020}},
}