Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes
(2007) In Autoimmunity 40(7). p.5-540- Abstract
BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.
AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.
SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n =... (More)
BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.
AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.
SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.
RESULTS: The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH < 7.1, p = 0.0328) and high ICA titres ( > or = 20 JDF units, p < 0.05).
CONCLUSIONS: Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed.
(Less)
- author
- publishing date
- 2007-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adolescent, Autoantibodies/blood, Child, Child, Preschool, Cuba, Diabetes Mellitus, Type 1/blood, Diabetic Ketoacidosis/blood, Enterovirus, Enterovirus Infections/blood, Europe, Female, Humans, Incidence, Infant, Insulin-Secreting Cells/immunology, Male, RNA, Viral/blood
- in
- Autoimmunity
- volume
- 40
- issue
- 7
- pages
- 5 - 540
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:17966045
- scopus:39049104346
- ISSN
- 0891-6934
- DOI
- 10.1080/08916930701523429
- language
- English
- LU publication?
- no
- id
- 470f41e6-ffaa-4baa-b654-983da9be6287
- date added to LUP
- 2019-07-08 14:46:51
- date last changed
- 2024-01-31 03:08:48
@article{470f41e6-ffaa-4baa-b654-983da9be6287,
abstract = {{<p>BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.</p><p>AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.</p><p>SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.</p><p>RESULTS: The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH < 7.1, p = 0.0328) and high ICA titres ( > or = 20 JDF units, p < 0.05).</p><p>CONCLUSIONS: Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed.</p>}},
author = {{Sarmiento, Luis and Cabrera-Rode, Eduardo and Lekuleni, Lister and Cuba, Ileana and Molina, Gisela and Fonseca, Magile and Heng-Hung, Lai and Borroto, Abel Diaz and Gonzalez, Pedro and Mas-Lago, Pedro and Diaz-Horta, Oscar}},
issn = {{0891-6934}},
keywords = {{Adolescent; Autoantibodies/blood; Child; Child, Preschool; Cuba; Diabetes Mellitus, Type 1/blood; Diabetic Ketoacidosis/blood; Enterovirus; Enterovirus Infections/blood; Europe; Female; Humans; Incidence; Infant; Insulin-Secreting Cells/immunology; Male; RNA, Viral/blood}},
language = {{eng}},
number = {{7}},
pages = {{5--540}},
publisher = {{Taylor & Francis}},
series = {{Autoimmunity}},
title = {{Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes}},
url = {{http://dx.doi.org/10.1080/08916930701523429}},
doi = {{10.1080/08916930701523429}},
volume = {{40}},
year = {{2007}},
}