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Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes

Sarmiento, Luis LU ; Cabrera-Rode, Eduardo ; Lekuleni, Lister ; Cuba, Ileana ; Molina, Gisela ; Fonseca, Magile ; Heng-Hung, Lai ; Borroto, Abel Diaz ; Gonzalez, Pedro and Mas-Lago, Pedro , et al. (2007) In Autoimmunity 40(7). p.5-540
Abstract

BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.

AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.

SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n =... (More)

BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.

AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.

SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.

RESULTS: The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH < 7.1, p = 0.0328) and high ICA titres ( > or = 20 JDF units, p < 0.05).

CONCLUSIONS: Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed.

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keywords
Adolescent, Autoantibodies/blood, Child, Child, Preschool, Cuba, Diabetes Mellitus, Type 1/blood, Diabetic Ketoacidosis/blood, Enterovirus, Enterovirus Infections/blood, Europe, Female, Humans, Incidence, Infant, Insulin-Secreting Cells/immunology, Male, RNA, Viral/blood
in
Autoimmunity
volume
40
issue
7
pages
5 - 540
publisher
Taylor & Francis
external identifiers
  • pmid:17966045
  • scopus:39049104346
ISSN
0891-6934
DOI
10.1080/08916930701523429
language
English
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no
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470f41e6-ffaa-4baa-b654-983da9be6287
date added to LUP
2019-07-08 14:46:51
date last changed
2019-11-20 05:50:09
@article{470f41e6-ffaa-4baa-b654-983da9be6287,
  abstract     = {<p>BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.</p><p>AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.</p><p>SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.</p><p>RESULTS: The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH &lt; 7.1, p = 0.0328) and high ICA titres ( &gt; or = 20 JDF units, p &lt; 0.05).</p><p>CONCLUSIONS: Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed.</p>},
  author       = {Sarmiento, Luis and Cabrera-Rode, Eduardo and Lekuleni, Lister and Cuba, Ileana and Molina, Gisela and Fonseca, Magile and Heng-Hung, Lai and Borroto, Abel Diaz and Gonzalez, Pedro and Mas-Lago, Pedro and Diaz-Horta, Oscar},
  issn         = {0891-6934},
  language     = {eng},
  number       = {7},
  pages        = {5--540},
  publisher    = {Taylor & Francis},
  series       = {Autoimmunity},
  title        = {Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes},
  url          = {http://dx.doi.org/10.1080/08916930701523429},
  doi          = {10.1080/08916930701523429},
  volume       = {40},
  year         = {2007},
}