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Dark-adapted spinach thylakoid protein heterogeneity offers insights into the photosystem II repair cycle

Suorsa, Marjaana; Rantala, Marjaana; Danielsson, Ravi LU ; Jarvi, Sari; Paakkarinen, Virpi; Schroder, Wolfgang P.; Styring, Stenbjorn; Mamedov, Fikret and Aro, Eva-Mari (2014) In Biochimica et Biophysica Acta - Bioenergetics 1837(9). p.1463-1471
Abstract
In higher plants, thylakoid membrane protein complexes show lateral heterogeneity in their distribution: photosystem (PS) II complexes are mostly located in grana stacks, whereas PSI and adenosine triphosphate (ATP) synthase are mostly found in the stroma-exposed thylakoids. However, recent research has revealed strong dynamics in distribution of photosystems and their light harvesting antenna along the thylakoid membrane. Here, the dark-adapted spinach (Spinacia oleracea L.) thylakoid network was mechanically fragmented and the composition of distinct PSII-related proteins in various thylakoid subdomains was analyzed in order to get more insights into the composition and localization of various PSII subcomplexes and auxiliary proteins... (More)
In higher plants, thylakoid membrane protein complexes show lateral heterogeneity in their distribution: photosystem (PS) II complexes are mostly located in grana stacks, whereas PSI and adenosine triphosphate (ATP) synthase are mostly found in the stroma-exposed thylakoids. However, recent research has revealed strong dynamics in distribution of photosystems and their light harvesting antenna along the thylakoid membrane. Here, the dark-adapted spinach (Spinacia oleracea L.) thylakoid network was mechanically fragmented and the composition of distinct PSII-related proteins in various thylakoid subdomains was analyzed in order to get more insights into the composition and localization of various PSII subcomplexes and auxiliary proteins during the PSII repair cycle. Most of the PSII subunits followed rather equal distribution with roughly 70% of the proteins located collectively in the grana thylakoids and grana margins; however, the low molecular mass subunits PsbW and PsbX as well as the PsbS proteins were found to be more exclusively located in grana thylakoids. The auxiliary proteins assisting in repair cycle of PSII were mostly located in stroma-exposed thylakoids, with the exception of THYLAKOID LUMEN PROTEIN OF 18.3 (TLP18.3), which was more evenly distributed between the grana and stroma thylakoids. The TL29 protein was present exclusively in grana thylakoids. Intriguingly, PROTON GRADIENT REGULATIONS (PGR5) was found to be distributed quite evenly between grana and stroma thylakoids, whereas PGR5-LIKE PHOTOSYNTHETIC PHENOTYPE1 (PGRL1) was highly enriched in the stroma thylakoids and practically missing from the grana cores. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. (C) 2013 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PGRS, PGRL1, Photosystem II, PsbS, PsbW, PsbX
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1837
issue
9
pages
1463 - 1471
publisher
Elsevier
external identifiers
  • wos:000341474300011
  • scopus:84906317542
ISSN
0005-2728
DOI
10.1016/j.bbabio.2013.11.014
language
English
LU publication?
yes
id
32aea78f-a4c5-4413-8b47-436edae29526 (old id 4713920)
date added to LUP
2014-10-27 11:02:37
date last changed
2017-09-10 04:18:43
@article{32aea78f-a4c5-4413-8b47-436edae29526,
  abstract     = {In higher plants, thylakoid membrane protein complexes show lateral heterogeneity in their distribution: photosystem (PS) II complexes are mostly located in grana stacks, whereas PSI and adenosine triphosphate (ATP) synthase are mostly found in the stroma-exposed thylakoids. However, recent research has revealed strong dynamics in distribution of photosystems and their light harvesting antenna along the thylakoid membrane. Here, the dark-adapted spinach (Spinacia oleracea L.) thylakoid network was mechanically fragmented and the composition of distinct PSII-related proteins in various thylakoid subdomains was analyzed in order to get more insights into the composition and localization of various PSII subcomplexes and auxiliary proteins during the PSII repair cycle. Most of the PSII subunits followed rather equal distribution with roughly 70% of the proteins located collectively in the grana thylakoids and grana margins; however, the low molecular mass subunits PsbW and PsbX as well as the PsbS proteins were found to be more exclusively located in grana thylakoids. The auxiliary proteins assisting in repair cycle of PSII were mostly located in stroma-exposed thylakoids, with the exception of THYLAKOID LUMEN PROTEIN OF 18.3 (TLP18.3), which was more evenly distributed between the grana and stroma thylakoids. The TL29 protein was present exclusively in grana thylakoids. Intriguingly, PROTON GRADIENT REGULATIONS (PGR5) was found to be distributed quite evenly between grana and stroma thylakoids, whereas PGR5-LIKE PHOTOSYNTHETIC PHENOTYPE1 (PGRL1) was highly enriched in the stroma thylakoids and practically missing from the grana cores. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. (C) 2013 Elsevier B.V. All rights reserved.},
  author       = {Suorsa, Marjaana and Rantala, Marjaana and Danielsson, Ravi and Jarvi, Sari and Paakkarinen, Virpi and Schroder, Wolfgang P. and Styring, Stenbjorn and Mamedov, Fikret and Aro, Eva-Mari},
  issn         = {0005-2728},
  keyword      = {PGRS,PGRL1,Photosystem II,PsbS,PsbW,PsbX},
  language     = {eng},
  number       = {9},
  pages        = {1463--1471},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Dark-adapted spinach thylakoid protein heterogeneity offers insights into the photosystem II repair cycle},
  url          = {http://dx.doi.org/10.1016/j.bbabio.2013.11.014},
  volume       = {1837},
  year         = {2014},
}