In vitro toxicity of biomaterials determined with cell density, total protein, cell cycle distribution and adenine nucleotides
(1993) In Artificial Cells, Blood Substitutes, and Biotechnology 21(1). p.63-70- Abstract
- Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until... (More)
- Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107064
- author
- Wieslander, Anders P ; Nordin, Marika K ; Hansson, Björn ; Baldetorp, Bo LU and Kjellstrand, Per T T
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Artificial Cells, Blood Substitutes, and Biotechnology
- volume
- 21
- issue
- 1
- pages
- 63 - 70
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:8461437
- scopus:0027406647
- ISSN
- 1055-7172
- DOI
- 10.3109/10731199309118297
- language
- English
- LU publication?
- yes
- id
- 472cd146-c1b9-4950-9016-ee935d86ee0a (old id 1107064)
- date added to LUP
- 2016-04-01 16:14:27
- date last changed
- 2021-01-03 03:27:07
@article{472cd146-c1b9-4950-9016-ee935d86ee0a,
abstract = {{Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity.}},
author = {{Wieslander, Anders P and Nordin, Marika K and Hansson, Björn and Baldetorp, Bo and Kjellstrand, Per T T}},
issn = {{1055-7172}},
language = {{eng}},
number = {{1}},
pages = {{63--70}},
publisher = {{Informa Healthcare}},
series = {{Artificial Cells, Blood Substitutes, and Biotechnology}},
title = {{In vitro toxicity of biomaterials determined with cell density, total protein, cell cycle distribution and adenine nucleotides}},
url = {{http://dx.doi.org/10.3109/10731199309118297}},
doi = {{10.3109/10731199309118297}},
volume = {{21}},
year = {{1993}},
}