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Co-ordination of cell cycle, migration and stem cell-like activity in breast cancer.

Lamb, Rebecca; Lisanti, Michael P; Clarke, Robert B and Landberg, Göran LU (2014) In Oncotarget 5(17). p.7833-7842
Abstract
Migration, proliferation and stem cell-like activity are all key cellular characteristics which aid the formation and progression of breast cancer, in addition to involvement in treatment resistance. Many current therapies aim to target tumour proliferation, and although successful, mortality rates in breast cancer remain significant. Our main objectives were to investigate the relationship between proliferation, migration and stem cell-like activity in breast cancer. We used a panel of cell lines and primary human breast cancer samples to assess the relationship between migration, proliferation and stem cells. We performed live cell sorting according to cell cycle (Hoechst-33324) and in combination with stem-cell markers (CD44/CD24/ESA)... (More)
Migration, proliferation and stem cell-like activity are all key cellular characteristics which aid the formation and progression of breast cancer, in addition to involvement in treatment resistance. Many current therapies aim to target tumour proliferation, and although successful, mortality rates in breast cancer remain significant. Our main objectives were to investigate the relationship between proliferation, migration and stem cell-like activity in breast cancer. We used a panel of cell lines and primary human breast cancer samples to assess the relationship between migration, proliferation and stem cells. We performed live cell sorting according to cell cycle (Hoechst-33324) and in combination with stem-cell markers (CD44/CD24/ESA) followed by assessment of migration and stem cell activity (mammosphere formation). We identified an inverse relationship between proliferation and migration/stem cell-like activity. G0/1 cells showed increased migration and mammosphere formation. Furthermore we identified a subpopulation of low proliferative stem-like cells (CD44+/24lo/ESA+) with increased migration and mammosphere formation that are specifically inhibited by Dickkopf 1 (DKK1) and Dibenzazepine (DBZ) known stem-cell inhibitors. These data show the co-ordination of migration, proliferation and stem cell activity in breast cancer, and has identified a sub-population of stem-like cells, greatly adding to our understanding of the complex nature of stem cell biology. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Oncotarget
volume
5
issue
17
pages
7833 - 7842
publisher
Impact Journals, LLC
external identifiers
  • pmid:25277201
  • wos:000348029800048
  • scopus:84907482174
ISSN
1949-2553
language
English
LU publication?
yes
id
918d90d1-8e7a-4887-9d12-d32449b88633 (old id 4738521)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25277201?dopt=Abstract
date added to LUP
2014-11-04 20:07:12
date last changed
2017-05-28 04:08:36
@article{918d90d1-8e7a-4887-9d12-d32449b88633,
  abstract     = {Migration, proliferation and stem cell-like activity are all key cellular characteristics which aid the formation and progression of breast cancer, in addition to involvement in treatment resistance. Many current therapies aim to target tumour proliferation, and although successful, mortality rates in breast cancer remain significant. Our main objectives were to investigate the relationship between proliferation, migration and stem cell-like activity in breast cancer. We used a panel of cell lines and primary human breast cancer samples to assess the relationship between migration, proliferation and stem cells. We performed live cell sorting according to cell cycle (Hoechst-33324) and in combination with stem-cell markers (CD44/CD24/ESA) followed by assessment of migration and stem cell activity (mammosphere formation). We identified an inverse relationship between proliferation and migration/stem cell-like activity. G0/1 cells showed increased migration and mammosphere formation. Furthermore we identified a subpopulation of low proliferative stem-like cells (CD44+/24lo/ESA+) with increased migration and mammosphere formation that are specifically inhibited by Dickkopf 1 (DKK1) and Dibenzazepine (DBZ) known stem-cell inhibitors. These data show the co-ordination of migration, proliferation and stem cell activity in breast cancer, and has identified a sub-population of stem-like cells, greatly adding to our understanding of the complex nature of stem cell biology.},
  author       = {Lamb, Rebecca and Lisanti, Michael P and Clarke, Robert B and Landberg, Göran},
  issn         = {1949-2553},
  language     = {eng},
  number       = {17},
  pages        = {7833--7842},
  publisher    = {Impact Journals, LLC},
  series       = {Oncotarget},
  title        = {Co-ordination of cell cycle, migration and stem cell-like activity in breast cancer.},
  volume       = {5},
  year         = {2014},
}