Proteomic characterization of plasma in Lemierre's syndrome
(2024) In Thrombosis and Haemostasis 124(05). p.432-440- Abstract
Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of... (More)
Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of differentially expressed proteins were defined based on a literature search focused on previous associations with thrombosis. Results Eight patients with Lemierre's syndrome and 15 with other severe infections were compared. Here, 20/449 identified proteins were differentially expressed between the groups. Of these, 14/20 had functions previously associated with thrombosis. 12/14 had a suggested prothrombotic effect in Lemierre's syndrome, whereas 2/14 had a suggested antithrombotic effect. Conclusion Proteins involved in several thrombogenic pathways were differentially expressed in Lemierre's syndrome compared to other severe infections. Among identified proteins, several were associated with endothelial damage, platelet activation and degranulation and warrant further targeted studies.
(Less)
- author
- Nygren, David
LU
; Torisson, Gustav LU
; Happonen, Lotta LU ; Mellhammar, Lisa LU ; Linder, Adam LU ; Elf, Johan LU ; Yan, Hong LU ; Welinder, Charlotte LU and Holm, Karin LU
- organization
-
- Translational infection medicine (research group)
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- Clinical infection medicine (research group)
- Structural Infection Medicine (STRIME) (research group)
- BioMS (research group)
- Translational Sepsis research (research group)
- Heparin bindning protein in cardiothoracic surgery (research group)
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- publishing date
- 2024
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fusobacterium necrophorum, Lemierre s syndrome, Mass spectrometr, Septic thrombophlebitis, Thrombogenesis
- in
- Thrombosis and Haemostasis
- volume
- 124
- issue
- 05
- pages
- 9 pages
- publisher
- Schattauer GmbH
- external identifiers
-
- pmid:37857346
- scopus:85174955037
- ISSN
- 0340-6245
- DOI
- 10.1055/a-2195-3927
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2023 Georg Thieme Verlag. All rights reserved.
- id
- 474bb3ae-1a9f-47c9-ba9d-9e1d2afe789d
- date added to LUP
- 2023-11-19 08:27:53
- date last changed
- 2025-02-06 23:43:29
@article{474bb3ae-1a9f-47c9-ba9d-9e1d2afe789d, abstract = {{<p>Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of differentially expressed proteins were defined based on a literature search focused on previous associations with thrombosis. Results Eight patients with Lemierre's syndrome and 15 with other severe infections were compared. Here, 20/449 identified proteins were differentially expressed between the groups. Of these, 14/20 had functions previously associated with thrombosis. 12/14 had a suggested prothrombotic effect in Lemierre's syndrome, whereas 2/14 had a suggested antithrombotic effect. Conclusion Proteins involved in several thrombogenic pathways were differentially expressed in Lemierre's syndrome compared to other severe infections. Among identified proteins, several were associated with endothelial damage, platelet activation and degranulation and warrant further targeted studies.</p>}}, author = {{Nygren, David and Torisson, Gustav and Happonen, Lotta and Mellhammar, Lisa and Linder, Adam and Elf, Johan and Yan, Hong and Welinder, Charlotte and Holm, Karin}}, issn = {{0340-6245}}, keywords = {{Fusobacterium necrophorum; Lemierre s syndrome; Mass spectrometr; Septic thrombophlebitis; Thrombogenesis}}, language = {{eng}}, number = {{05}}, pages = {{432--440}}, publisher = {{Schattauer GmbH}}, series = {{Thrombosis and Haemostasis}}, title = {{Proteomic characterization of plasma in Lemierre's syndrome}}, url = {{http://dx.doi.org/10.1055/a-2195-3927}}, doi = {{10.1055/a-2195-3927}}, volume = {{124}}, year = {{2024}}, }