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Proteomic characterization of plasma in Lemierre's syndrome

Nygren, David LU orcid ; Torisson, Gustav LU orcid ; Happonen, Lotta LU ; Mellhammar, Lisa LU ; Linder, Adam LU ; Elf, Johan LU ; Yan, Hong LU ; Welinder, Charlotte LU and Holm, Karin LU (2023) In Thrombosis and Haemostasis
Abstract

Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of... (More)

Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of differentially expressed proteins were defined based on a literature search focused on previous associations with thrombosis. Results Eight patients with Lemierre's syndrome and 15 with other severe infections were compared. Here, 20/449 identified proteins were differentially expressed between the groups. Of these, 14/20 had functions previously associated with thrombosis. 12/14 had a suggested prothrombotic effect in Lemierre's syndrome, whereas 2/14 had a suggested antithrombotic effect. Conclusion Proteins involved in several thrombogenic pathways were differentially expressed in Lemierre's syndrome compared to other severe infections. Among identified proteins, several were associated with endothelial damage, platelet activation and degranulation and warrant further targeted studies.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Fusobacterium necrophorum, Lemierre s syndrome, Mass spectrometr, Septic thrombophlebitis, Thrombogenesis
in
Thrombosis and Haemostasis
publisher
Schattauer GmbH
external identifiers
  • pmid:37857346
  • scopus:85174955037
ISSN
0340-6245
DOI
10.1055/a-2195-3927
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2023 Georg Thieme Verlag. All rights reserved.
id
474bb3ae-1a9f-47c9-ba9d-9e1d2afe789d
date added to LUP
2023-11-19 08:27:53
date last changed
2024-04-16 15:07:07
@article{474bb3ae-1a9f-47c9-ba9d-9e1d2afe789d,
  abstract     = {{<p>Background The underlying mechanisms of thrombosis in Lemierre's syndrome and other septic thrombophlebitis are incompletely understood. Therefore, in this case-control study we aimed to generate hypotheses on its pathogenesis by studying the plasma proteome in patients with these conditions. Methods All patients with Lemierre's syndrome in the Skåne Region, Sweden were enrolled prospectively during 2017-2021 as cases. Age-matched patients with other severe infections were enrolled as controls. Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. Differentially expressed proteins in Lemierre's syndrome vs. other severe infections were highlighted. Functions of differentially expressed proteins were defined based on a literature search focused on previous associations with thrombosis. Results Eight patients with Lemierre's syndrome and 15 with other severe infections were compared. Here, 20/449 identified proteins were differentially expressed between the groups. Of these, 14/20 had functions previously associated with thrombosis. 12/14 had a suggested prothrombotic effect in Lemierre's syndrome, whereas 2/14 had a suggested antithrombotic effect. Conclusion Proteins involved in several thrombogenic pathways were differentially expressed in Lemierre's syndrome compared to other severe infections. Among identified proteins, several were associated with endothelial damage, platelet activation and degranulation and warrant further targeted studies.</p>}},
  author       = {{Nygren, David and Torisson, Gustav and Happonen, Lotta and Mellhammar, Lisa and Linder, Adam and Elf, Johan and Yan, Hong and Welinder, Charlotte and Holm, Karin}},
  issn         = {{0340-6245}},
  keywords     = {{Fusobacterium necrophorum; Lemierre s syndrome; Mass spectrometr; Septic thrombophlebitis; Thrombogenesis}},
  language     = {{eng}},
  publisher    = {{Schattauer GmbH}},
  series       = {{Thrombosis and Haemostasis}},
  title        = {{Proteomic characterization of plasma in Lemierre's syndrome}},
  url          = {{http://dx.doi.org/10.1055/a-2195-3927}},
  doi          = {{10.1055/a-2195-3927}},
  year         = {{2023}},
}