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Telomerase in yeast

Cohn, Marita LU and Blackburn, E.H. (1995) In Science 269(5222). p.396-400
Abstract
The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae-with regular and irregular telomeric sequences, respectively-have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Science
volume
269
issue
5222
pages
396 - 400
publisher
The American Association for the Advancement of Science
external identifiers
  • scopus:0029155795
ISSN
1095-9203
DOI
10.1126/science.7618104
language
English
LU publication?
no
id
c5f8eb2a-8720-4002-80f7-eba064104149 (old id 4754351)
date added to LUP
2014-11-06 11:28:25
date last changed
2017-07-30 04:42:35
@article{c5f8eb2a-8720-4002-80f7-eba064104149,
  abstract     = {The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae-with regular and irregular telomeric sequences, respectively-have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.},
  author       = {Cohn, Marita and Blackburn, E.H.},
  issn         = {1095-9203},
  language     = {eng},
  number       = {5222},
  pages        = {396--400},
  publisher    = {The American Association for the Advancement of Science},
  series       = {Science},
  title        = {Telomerase in yeast},
  url          = {http://dx.doi.org/10.1126/science.7618104},
  volume       = {269},
  year         = {1995},
}