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Variability observed in mechano-regulated in vivo tissue differentiation can be explained by variation in cell mechano-sensitivity

Khayyeri, Hanifeh LU ; Checa, Sara; Tägil, Magnus LU ; Aspenberg, Per and Prendergast, Patrick J. (2011) In Journal of Biomechanics 44(6). p.1051-1058
Abstract
Computational simulations of tissue differentiation have been able to capture the main aspects of tissue formation/regeneration observed in animal experiments—except for the considerable degree of variability reported. Understanding and modelling the source of this variability is crucial if computational tools are to be developed for clinical applications. The objective of this study was to test the hypothesis that differences in cell mechano-sensitivity between individuals can explain the variability of tissue differentiation patterns observed experimentally. Simulations of an experiment of tissue differentiation in a mechanically loaded bone chamber were performed. Finite element analysis was used to determine the biophysical... (More)
Computational simulations of tissue differentiation have been able to capture the main aspects of tissue formation/regeneration observed in animal experiments—except for the considerable degree of variability reported. Understanding and modelling the source of this variability is crucial if computational tools are to be developed for clinical applications. The objective of this study was to test the hypothesis that differences in cell mechano-sensitivity between individuals can explain the variability of tissue differentiation patterns observed experimentally. Simulations of an experiment of tissue differentiation in a mechanically loaded bone chamber were performed. Finite element analysis was used to determine the biophysical environment, and a lattice-modelling approach was used to simulate cell activity. Differences in cell mechano-sensitivity among individuals were modelled as differences in cell activity rates, with the activation of cell activities regulated by the mechanical environment. Predictions of the tissue distribution in the chambers produced the two different classes of results found experimentally: (i) chambers with a layer of bone across the chamber covered by a layer of cartilage on top and (ii) chambers with almost no bone, mainly fibrous tissue and small islands of cartilage. This indicates that the differing cellular response to the mechanical environment (i.e., subject-specific mechano-sensitivity) could be a reason for the different outcomes found when implants (or tissue engineered constructs) are used in a population. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mechano-regulation, Mechanobiology, Inter-specimen variability, FE-model, Lattice model
in
Journal of Biomechanics
volume
44
issue
6
pages
1051 - 1058
publisher
Elsevier
external identifiers
  • scopus:79953029767
  • wos:000290187500010
ISSN
1873-2380
DOI
10.1016/j.jbiomech.2011.02.003
language
English
LU publication?
yes
id
c6f9b4dc-4ee2-47e2-9581-1184130c58e4 (old id 4778780)
date added to LUP
2011-07-01 09:28:15
date last changed
2017-04-09 03:02:43
@article{c6f9b4dc-4ee2-47e2-9581-1184130c58e4,
  abstract     = {Computational simulations of tissue differentiation have been able to capture the main aspects of tissue formation/regeneration observed in animal experiments—except for the considerable degree of variability reported. Understanding and modelling the source of this variability is crucial if computational tools are to be developed for clinical applications. The objective of this study was to test the hypothesis that differences in cell mechano-sensitivity between individuals can explain the variability of tissue differentiation patterns observed experimentally. Simulations of an experiment of tissue differentiation in a mechanically loaded bone chamber were performed. Finite element analysis was used to determine the biophysical environment, and a lattice-modelling approach was used to simulate cell activity. Differences in cell mechano-sensitivity among individuals were modelled as differences in cell activity rates, with the activation of cell activities regulated by the mechanical environment. Predictions of the tissue distribution in the chambers produced the two different classes of results found experimentally: (i) chambers with a layer of bone across the chamber covered by a layer of cartilage on top and (ii) chambers with almost no bone, mainly fibrous tissue and small islands of cartilage. This indicates that the differing cellular response to the mechanical environment (i.e., subject-specific mechano-sensitivity) could be a reason for the different outcomes found when implants (or tissue engineered constructs) are used in a population.},
  author       = {Khayyeri, Hanifeh and Checa, Sara and Tägil, Magnus and Aspenberg, Per and Prendergast, Patrick J.},
  issn         = {1873-2380},
  keyword      = {Mechano-regulation,Mechanobiology,Inter-specimen variability,FE-model,Lattice model},
  language     = {eng},
  number       = {6},
  pages        = {1051--1058},
  publisher    = {Elsevier},
  series       = {Journal of Biomechanics},
  title        = {Variability observed in mechano-regulated in vivo tissue differentiation can be explained by variation in cell mechano-sensitivity},
  url          = {http://dx.doi.org/10.1016/j.jbiomech.2011.02.003},
  volume       = {44},
  year         = {2011},
}