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Cell cycle synchronization of embryonic stem cells : Effect of serum deprivation on the differentiation of embryonic bodies in vitro

Zhang, Enming LU ; Li, Xiaolong ; Zhang, Shufang ; Chen, Liangqiang and Zheng, Xiaoxiang (2005) In Biochemical and Biophysical Research Communications 333(4). p.1171-1177
Abstract

Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle... (More)

Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle successfully after serum supply. EBs formed from synchronized ES cells have higher totipotency capability to differentiate into functional neuronal cells than EBs formed from unsynchronized ES cells. Our study provides a method for ES treatment before cell transplantation that possibly helps to decrease the rate of cell death after transplantation.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell synchronization, Differentiation, Embryonic bodies, Embryonic stem cells, Serum deprivation
in
Biochemical and Biophysical Research Communications
volume
333
issue
4
pages
1171 - 1177
publisher
Elsevier
external identifiers
  • scopus:21344451374
  • pmid:15975550
ISSN
0006-291X
DOI
10.1016/j.bbrc.2005.05.200
language
English
LU publication?
no
id
478b82cc-5de2-4428-9111-c100fe03c23e
date added to LUP
2018-11-14 14:21:58
date last changed
2024-03-02 11:49:08
@article{478b82cc-5de2-4428-9111-c100fe03c23e,
  abstract     = {{<p>Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle successfully after serum supply. EBs formed from synchronized ES cells have higher totipotency capability to differentiate into functional neuronal cells than EBs formed from unsynchronized ES cells. Our study provides a method for ES treatment before cell transplantation that possibly helps to decrease the rate of cell death after transplantation.</p>}},
  author       = {{Zhang, Enming and Li, Xiaolong and Zhang, Shufang and Chen, Liangqiang and Zheng, Xiaoxiang}},
  issn         = {{0006-291X}},
  keywords     = {{Cell synchronization; Differentiation; Embryonic bodies; Embryonic stem cells; Serum deprivation}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1171--1177}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Cell cycle synchronization of embryonic stem cells : Effect of serum deprivation on the differentiation of embryonic bodies in vitro}},
  url          = {{http://dx.doi.org/10.1016/j.bbrc.2005.05.200}},
  doi          = {{10.1016/j.bbrc.2005.05.200}},
  volume       = {{333}},
  year         = {{2005}},
}