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Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans

Burra, Dharani Dhar; Berkowitz, Oliver; Hedley, Pete E.; Morris, Jenny; Resjoe, Svante; Levander, Fredrik LU ; Liljeroth, Erland; Andreasson, Erik and Alexandersson, Erik (2014) In BMC Plant Biology 14.
Abstract
Background: Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated the transcriptome of Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative proteomics after foliar application of the protective agent phosphite. We also studied the distribution of phosphite in planta after application and tested transgenic potato lines with impaired in salicylic and jasmonic acid signaling. Results: Phosphite had a rapid and transient effect on the transcriptome, with a clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, whereas protection was observed throughout all time points tested. In contrast, 67 secretome proteins predominantly... (More)
Background: Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated the transcriptome of Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative proteomics after foliar application of the protective agent phosphite. We also studied the distribution of phosphite in planta after application and tested transgenic potato lines with impaired in salicylic and jasmonic acid signaling. Results: Phosphite had a rapid and transient effect on the transcriptome, with a clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, whereas protection was observed throughout all time points tested. In contrast, 67 secretome proteins predominantly associated with cell-wall processes and defense changed in abundance at 48 h after treatment. Transcripts associated with defense, wounding, and oxidative stress constituted the core of the phosphite response. We also observed changes in primary metabolism and cell wall-related processes. These changes were shown not to be due to phosphate depletion or acidification caused by phosphite treatment. Of the phosphite-regulated transcripts 40% also changed with beta-aminobutyric acid (BABA) as an elicitor, while the defence gene PR1 was only up-regulated by BABA. Although phosphite was shown to be distributed in planta to parts not directly exposed to phosphite, no protection in leaves without direct foliar application was observed. Furthermore, the analysis of transgenic potato lines indicated that the phosphite-mediated resistance was independent of the plant hormones salicylic and jasmonic acid. Conclusions: Our study suggests that a rapid phosphite-triggered response is important to confer long-lasting resistance against P. infestans and gives molecular understanding of its successful field applications. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Phosphite, Late blight, Phytophthora infestans, Potato, Secretome, Microarray, Induced resistance, Transgenic lines
in
BMC Plant Biology
volume
14
publisher
BioMed Central
external identifiers
  • wos:000342782300001
  • scopus:84908113890
ISSN
1471-2229
DOI
10.1186/s12870-014-0254-y
language
English
LU publication?
yes
id
68d45c81-3a63-4614-9d8e-af6fdfc598ef (old id 4796328)
date added to LUP
2014-11-24 16:37:14
date last changed
2017-11-19 03:52:22
@article{68d45c81-3a63-4614-9d8e-af6fdfc598ef,
  abstract     = {Background: Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated the transcriptome of Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative proteomics after foliar application of the protective agent phosphite. We also studied the distribution of phosphite in planta after application and tested transgenic potato lines with impaired in salicylic and jasmonic acid signaling. Results: Phosphite had a rapid and transient effect on the transcriptome, with a clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, whereas protection was observed throughout all time points tested. In contrast, 67 secretome proteins predominantly associated with cell-wall processes and defense changed in abundance at 48 h after treatment. Transcripts associated with defense, wounding, and oxidative stress constituted the core of the phosphite response. We also observed changes in primary metabolism and cell wall-related processes. These changes were shown not to be due to phosphate depletion or acidification caused by phosphite treatment. Of the phosphite-regulated transcripts 40% also changed with beta-aminobutyric acid (BABA) as an elicitor, while the defence gene PR1 was only up-regulated by BABA. Although phosphite was shown to be distributed in planta to parts not directly exposed to phosphite, no protection in leaves without direct foliar application was observed. Furthermore, the analysis of transgenic potato lines indicated that the phosphite-mediated resistance was independent of the plant hormones salicylic and jasmonic acid. Conclusions: Our study suggests that a rapid phosphite-triggered response is important to confer long-lasting resistance against P. infestans and gives molecular understanding of its successful field applications.},
  articleno    = {254},
  author       = {Burra, Dharani Dhar and Berkowitz, Oliver and Hedley, Pete E. and Morris, Jenny and Resjoe, Svante and Levander, Fredrik and Liljeroth, Erland and Andreasson, Erik and Alexandersson, Erik},
  issn         = {1471-2229},
  keyword      = {Phosphite,Late blight,Phytophthora infestans,Potato,Secretome,Microarray,Induced resistance,Transgenic lines},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Plant Biology},
  title        = {Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans},
  url          = {http://dx.doi.org/10.1186/s12870-014-0254-y},
  volume       = {14},
  year         = {2014},
}