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Protein depositions on one hydrocephalus shunt and on fifteen temporary ventricular catheters

Lundberg, F. LU ; Tegenfeldt, J. O. LU ; Montelius, L. LU ; Ransjö, U. ; Appelgren, P. ; Siesjö, P. LU and Ljungh, Å LU (1997) In Acta Neurochirurgica 139(8). p.734-742
Abstract

Biomaterials are commonly used in modern medicine. Proteins are adsorbed to the surface of the biomaterial immediately after insertion. This report demonstrates the presence of adsorbed proteins in one infected cerebrospinal shunt from a child with hydrocephalus and on fifteen temporary ventricular catheters from adult patients with spontaneous or traumatic brain injuries. Depositions of vitronectin, fibrinogen and thrombospondin-fibronectin to some extent - on the shunt surface was imaged by field-emission scanning electron microscopy. Vitronectin, fibronectin, fibrinogen, and thrombospondin on the ventricular catheters were shown with radio-actively labelled antibodies. Furthermore, protein adsorption from human cerebrospinal fluid to... (More)

Biomaterials are commonly used in modern medicine. Proteins are adsorbed to the surface of the biomaterial immediately after insertion. This report demonstrates the presence of adsorbed proteins in one infected cerebrospinal shunt from a child with hydrocephalus and on fifteen temporary ventricular catheters from adult patients with spontaneous or traumatic brain injuries. Depositions of vitronectin, fibrinogen and thrombospondin-fibronectin to some extent - on the shunt surface was imaged by field-emission scanning electron microscopy. Vitronectin, fibronectin, fibrinogen, and thrombospondin on the ventricular catheters were shown with radio-actively labelled antibodies. Furthermore, protein adsorption from human cerebrospinal fluid to heparinized and unheparinized polymers was studied under flowing conditions in vitro. On heparinized polymer, significantly reduced levels of vitronectin, fibronectin, and thrombospondin were exposed, as measured after 4 hours in vitro perfusion. After 24 hours perfusion, the differences in protein exposition between heparinized and unheparinized polymers were substantially reduced.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Protein, Shunt, Ventricular catheter
in
Acta Neurochirurgica
volume
139
issue
8
pages
734 - 742
publisher
Springer
external identifiers
  • pmid:9309288
  • scopus:0030807175
ISSN
0001-6268
DOI
10.1007/BF01420046
language
English
LU publication?
yes
id
47b27920-9dd5-4d5c-b6b1-cd5e09b6abe9
date added to LUP
2018-10-20 10:52:46
date last changed
2020-01-13 01:05:04
@article{47b27920-9dd5-4d5c-b6b1-cd5e09b6abe9,
  abstract     = {<p>Biomaterials are commonly used in modern medicine. Proteins are adsorbed to the surface of the biomaterial immediately after insertion. This report demonstrates the presence of adsorbed proteins in one infected cerebrospinal shunt from a child with hydrocephalus and on fifteen temporary ventricular catheters from adult patients with spontaneous or traumatic brain injuries. Depositions of vitronectin, fibrinogen and thrombospondin-fibronectin to some extent - on the shunt surface was imaged by field-emission scanning electron microscopy. Vitronectin, fibronectin, fibrinogen, and thrombospondin on the ventricular catheters were shown with radio-actively labelled antibodies. Furthermore, protein adsorption from human cerebrospinal fluid to heparinized and unheparinized polymers was studied under flowing conditions in vitro. On heparinized polymer, significantly reduced levels of vitronectin, fibronectin, and thrombospondin were exposed, as measured after 4 hours in vitro perfusion. After 24 hours perfusion, the differences in protein exposition between heparinized and unheparinized polymers were substantially reduced.</p>},
  author       = {Lundberg, F. and Tegenfeldt, J. O. and Montelius, L. and Ransjö, U. and Appelgren, P. and Siesjö, P. and Ljungh, Å},
  issn         = {0001-6268},
  language     = {eng},
  month        = {09},
  number       = {8},
  pages        = {734--742},
  publisher    = {Springer},
  series       = {Acta Neurochirurgica},
  title        = {Protein depositions on one hydrocephalus shunt and on fifteen temporary ventricular catheters},
  url          = {http://dx.doi.org/10.1007/BF01420046},
  doi          = {10.1007/BF01420046},
  volume       = {139},
  year         = {1997},
}