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HnRNP G prevents inclusion on the HPV16 L1 mRNAs of the central exon between splice sites SA3358 and SD3632

Yu, Haoran LU ; Gong, Lijing LU ; Wu, Chengjun LU ; Nilsson, Kersti LU ; Li-Wang, Xiaoze LU and Schwartz, Stefan LU (2018) In Journal of General Virology 99(3). p.328-343
Abstract

HPV16 late L1 mRNAs encode a short central exon that is located between HPV16 3′-splice site SA3358 and HPV16 5′-splice site SD3632. While SA3358 is used to produce both HPV16 early mRNAs encoding the E6 and E7 oncogenes, and late mRNAs encoding E4, L1 and L2, SD3632 is used exclusively to produce late L1 mRNA. We have previously identified an 8-nucleotide regulatory RNA element that is required for inclusion of the exon between SA3358 and SD3632 to produce L1 mRNAs at the expense of mRNAs polyadenylated at the HPV16 early polyadenylation signal pAE. Here we show that this HPV16 8-nucleotide splicing enhancer interacts with hnRNP G. Binding of hnRNP G to this element prevents inclusion of the exon between SA3358 and SD3632 on the HPV16... (More)

HPV16 late L1 mRNAs encode a short central exon that is located between HPV16 3′-splice site SA3358 and HPV16 5′-splice site SD3632. While SA3358 is used to produce both HPV16 early mRNAs encoding the E6 and E7 oncogenes, and late mRNAs encoding E4, L1 and L2, SD3632 is used exclusively to produce late L1 mRNA. We have previously identified an 8-nucleotide regulatory RNA element that is required for inclusion of the exon between SA3358 and SD3632 to produce L1 mRNAs at the expense of mRNAs polyadenylated at the HPV16 early polyadenylation signal pAE. Here we show that this HPV16 8-nucleotide splicing enhancer interacts with hnRNP G. Binding of hnRNP G to this element prevents inclusion of the exon between SA3358 and SD3632 on the HPV16 late L1 mRNAs. We concluded that hnRNP G has a splicing inhibitory role and that hnRNP G can control HPV16 mRNA splicing.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HnRNP G, Papillomavirus, Polyadenylation, Splicing, SR proteins
in
Journal of General Virology
volume
99
issue
3
article number
001019
pages
16 pages
publisher
Society for General Microbiology
external identifiers
  • pmid:29458523
  • scopus:85043762470
ISSN
0022-1317
DOI
10.1099/jgv.0.001019
language
English
LU publication?
yes
id
47dfd6b6-5b4a-41f2-ae5f-011fab693000
date added to LUP
2018-03-27 13:54:56
date last changed
2020-02-12 09:22:50
@article{47dfd6b6-5b4a-41f2-ae5f-011fab693000,
  abstract     = {<p>HPV16 late L1 mRNAs encode a short central exon that is located between HPV16 3′-splice site SA3358 and HPV16 5′-splice site SD3632. While SA3358 is used to produce both HPV16 early mRNAs encoding the E6 and E7 oncogenes, and late mRNAs encoding E4, L1 and L2, SD3632 is used exclusively to produce late L1 mRNA. We have previously identified an 8-nucleotide regulatory RNA element that is required for inclusion of the exon between SA3358 and SD3632 to produce L1 mRNAs at the expense of mRNAs polyadenylated at the HPV16 early polyadenylation signal pAE. Here we show that this HPV16 8-nucleotide splicing enhancer interacts with hnRNP G. Binding of hnRNP G to this element prevents inclusion of the exon between SA3358 and SD3632 on the HPV16 late L1 mRNAs. We concluded that hnRNP G has a splicing inhibitory role and that hnRNP G can control HPV16 mRNA splicing.</p>},
  author       = {Yu, Haoran and Gong, Lijing and Wu, Chengjun and Nilsson, Kersti and Li-Wang, Xiaoze and Schwartz, Stefan},
  issn         = {0022-1317},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {328--343},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {HnRNP G prevents inclusion on the HPV16 L1 mRNAs of the central exon between splice sites SA3358 and SD3632},
  url          = {http://dx.doi.org/10.1099/jgv.0.001019},
  doi          = {10.1099/jgv.0.001019},
  volume       = {99},
  year         = {2018},
}