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Acceleration of α-Synuclein Aggregation by Exosomes.

Grey, Marie; Dunning, Christopher LU ; Gaspar, Ricardo; Grey, Carl; Brundin, Patrik LU ; Sparr, Emma and Linse, Sara (2015) In Journal of Biological Chemistry 290(5). p.2969-2982
Abstract
Exosomes are small vesicles released from cells into extra-cellular space. We have isolated exosomes from neuroblastoma cells and investigated their influence on the aggregation of α-synuclein, a protein associated with Parkinson disease pathology. Using cryo-transmission electron microscopy of exosomes we found spherical unilamellar vesicles with a significant protein content, and Western blot analysis revealed that they contain, as expected, the proteins flotillin-1 and alix. Using thioflavin T fluorescence to monitor aggregation kinetics, we found that exosomes catalyze the process in a similar manner as low concentration of preformed α-synuclein fibrils. The exosomes reduce the lag time indicating that they provide catalytic... (More)
Exosomes are small vesicles released from cells into extra-cellular space. We have isolated exosomes from neuroblastoma cells and investigated their influence on the aggregation of α-synuclein, a protein associated with Parkinson disease pathology. Using cryo-transmission electron microscopy of exosomes we found spherical unilamellar vesicles with a significant protein content, and Western blot analysis revealed that they contain, as expected, the proteins flotillin-1 and alix. Using thioflavin T fluorescence to monitor aggregation kinetics, we found that exosomes catalyze the process in a similar manner as low concentration of preformed α-synuclein fibrils. The exosomes reduce the lag time indicating that they provide catalytic environments for nucleation. The catalytic effect of exosomes derived from naive cells and cells that over-express α-synuclein do not differ. Vesicles prepared from extracted exosome lipids accelerate aggregation, suggesting that the lipids in exosomes are sufficient for the catalytic effect to arise. Using mass spectrometry we found several phospholipid classes in the exosomes, including phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol and the gangliosides GM2 and GM3. Within each class, several species with different acyl chains were identified. We then prepared vesicles from corresponding pure lipids or defined mixtures, most of which were found to retard α-synuclein aggregation. As a striking exception, vesicles containing ganglioside lipids GM1 or GM3 accelerate the process. Understanding how α-synuclein interacts with biological membranes to promote neurological disease might lead to the identification of novel therapeutic targets. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
290
issue
5
pages
2969 - 2982
publisher
ASBMB
external identifiers
  • pmid:25425650
  • wos:000349310700035
  • scopus:84921915868
ISSN
1083-351X
DOI
10.1074/jbc.M114.585703
language
English
LU publication?
yes
id
f6f70537-a9a3-4f29-8a66-cf45d6d41401 (old id 4815975)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25425650?dopt=Abstract
date added to LUP
2014-12-04 09:01:08
date last changed
2017-11-05 03:13:49
@article{f6f70537-a9a3-4f29-8a66-cf45d6d41401,
  abstract     = {Exosomes are small vesicles released from cells into extra-cellular space. We have isolated exosomes from neuroblastoma cells and investigated their influence on the aggregation of α-synuclein, a protein associated with Parkinson disease pathology. Using cryo-transmission electron microscopy of exosomes we found spherical unilamellar vesicles with a significant protein content, and Western blot analysis revealed that they contain, as expected, the proteins flotillin-1 and alix. Using thioflavin T fluorescence to monitor aggregation kinetics, we found that exosomes catalyze the process in a similar manner as low concentration of preformed α-synuclein fibrils. The exosomes reduce the lag time indicating that they provide catalytic environments for nucleation. The catalytic effect of exosomes derived from naive cells and cells that over-express α-synuclein do not differ. Vesicles prepared from extracted exosome lipids accelerate aggregation, suggesting that the lipids in exosomes are sufficient for the catalytic effect to arise. Using mass spectrometry we found several phospholipid classes in the exosomes, including phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol and the gangliosides GM2 and GM3. Within each class, several species with different acyl chains were identified. We then prepared vesicles from corresponding pure lipids or defined mixtures, most of which were found to retard α-synuclein aggregation. As a striking exception, vesicles containing ganglioside lipids GM1 or GM3 accelerate the process. Understanding how α-synuclein interacts with biological membranes to promote neurological disease might lead to the identification of novel therapeutic targets.},
  author       = {Grey, Marie and Dunning, Christopher and Gaspar, Ricardo and Grey, Carl and Brundin, Patrik and Sparr, Emma and Linse, Sara},
  issn         = {1083-351X},
  language     = {eng},
  number       = {5},
  pages        = {2969--2982},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Acceleration of α-Synuclein Aggregation by Exosomes.},
  url          = {http://dx.doi.org/10.1074/jbc.M114.585703},
  volume       = {290},
  year         = {2015},
}