Hard sphere-like glass transition in eye lens α-crystallin solutions.
Foffi, Giuseppe ; Savin, Gabriela ; Bucciarelli, Saskia LU ; Dorsaz, Nicolas ; Thurston, George M ; Stradner, Anna LU and Schurtenberger, Peter LU
(2014)
In Proceedings of the National Academy of Sciences
111(47).
p.16748-16753
- Abstract
- We study the equilibrium liquid structure and dynamics of dilute and concentrated bovine eye lens α-crystallin solutions, using small-angle X-ray scattering, static and dynamic light scattering, viscometry, molecular dynamics simulations, and mode-coupling theory. We find that a polydisperse Percus-Yevick hard-sphere liquid-structure model accurately reproduces both static light scattering data and small-angle X-ray scattering liquid structure data from α-crystallin solutions over an extended range of protein concentrations up to 290 mg/mL or 49% vol fraction and up to ca. 330 mg/mL for static light scattering. The measured dynamic light scattering and viscosity properties are also consistent with those of hard-sphere colloids and show... (More)
- We study the equilibrium liquid structure and dynamics of dilute and concentrated bovine eye lens α-crystallin solutions, using small-angle X-ray scattering, static and dynamic light scattering, viscometry, molecular dynamics simulations, and mode-coupling theory. We find that a polydisperse Percus-Yevick hard-sphere liquid-structure model accurately reproduces both static light scattering data and small-angle X-ray scattering liquid structure data from α-crystallin solutions over an extended range of protein concentrations up to 290 mg/mL or 49% vol fraction and up to ca. 330 mg/mL for static light scattering. The measured dynamic light scattering and viscosity properties are also consistent with those of hard-sphere colloids and show power laws characteristic of an approach toward a glass transition at α-crystallin volume fractions near 58%. Dynamic light scattering at a volume fraction beyond the glass transition indicates formation of an arrested state. We further perform event-driven molecular dynamics simulations of polydisperse hard-sphere systems and use mode-coupling theory to compare the measured dynamic power laws with those of hard-sphere models. The static and dynamic data, simulations, and analysis show that aqueous eye lens α-crystallin solutions exhibit a glass transition at high concentrations that is similar to those found in hard-sphere colloidal systems. The α-crystallin glass transition could have implications for the molecular basis of presbyopia and the kinetics of molecular change during cataractogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4817023
- author
- Foffi, Giuseppe
; Savin, Gabriela
; Bucciarelli, Saskia
LU
; Dorsaz, Nicolas
; Thurston, George M
; Stradner, Anna
LU
and Schurtenberger, Peter
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences
- volume
- 111
- issue
- 47
- pages
- 16748 - 16753
- publisher
- National Academy of Sciences
- external identifiers
-
- pmid:25385638
- wos:000345662700035
- scopus:84912562512
- pmid:25385638
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.1406990111
- language
- English
- LU publication?
- yes
- id
- bd290540-ac13-4779-8658-129d811d50aa (old id 4817023)
- date added to LUP
- 2016-04-01 09:50:07
- date last changed
- 2022-04-11 23:17:19
@article{bd290540-ac13-4779-8658-129d811d50aa,
abstract = {{We study the equilibrium liquid structure and dynamics of dilute and concentrated bovine eye lens α-crystallin solutions, using small-angle X-ray scattering, static and dynamic light scattering, viscometry, molecular dynamics simulations, and mode-coupling theory. We find that a polydisperse Percus-Yevick hard-sphere liquid-structure model accurately reproduces both static light scattering data and small-angle X-ray scattering liquid structure data from α-crystallin solutions over an extended range of protein concentrations up to 290 mg/mL or 49% vol fraction and up to ca. 330 mg/mL for static light scattering. The measured dynamic light scattering and viscosity properties are also consistent with those of hard-sphere colloids and show power laws characteristic of an approach toward a glass transition at α-crystallin volume fractions near 58%. Dynamic light scattering at a volume fraction beyond the glass transition indicates formation of an arrested state. We further perform event-driven molecular dynamics simulations of polydisperse hard-sphere systems and use mode-coupling theory to compare the measured dynamic power laws with those of hard-sphere models. The static and dynamic data, simulations, and analysis show that aqueous eye lens α-crystallin solutions exhibit a glass transition at high concentrations that is similar to those found in hard-sphere colloidal systems. The α-crystallin glass transition could have implications for the molecular basis of presbyopia and the kinetics of molecular change during cataractogenesis.}},
author = {{Foffi, Giuseppe and Savin, Gabriela and Bucciarelli, Saskia and Dorsaz, Nicolas and Thurston, George M and Stradner, Anna and Schurtenberger, Peter}},
issn = {{1091-6490}},
language = {{eng}},
number = {{47}},
pages = {{16748--16753}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences}},
title = {{Hard sphere-like glass transition in eye lens α-crystallin solutions.}},
url = {{http://dx.doi.org/10.1073/pnas.1406990111}},
doi = {{10.1073/pnas.1406990111}},
volume = {{111}},
year = {{2014}},
}