Sensing Mechanism and Excited-State Dynamics of a Widely Used Intracellular Fluorescent pH Probe : pHrodo
(2023) In The Journal of Physical Chemistry Letters 14(46). p.10482-10488- Abstract
- The pHrodo with an “off–on” response to the changes of pH has been
widely used as a fluorescent pH probe for bioimaging. The fluorescence
off–on mechanism is fundamentally important for its application and
further development. Herein, the sensing mechanism, especially the
relevant excited-state dynamics, of pHrodo is investigated by
steady-state and time-resolved spectroscopy as well as quantum chemical
calculations, showing that pHrodo is best understood using the
bichromophore model. Its first excited state (S1) is a charge transfer state between two chromophores. From S1, pHrodo relaxes to its ground state (S0) via an ultrafast nonradiative process (∼0.5 ps), which... (More) - The pHrodo with an “off–on” response to the changes of pH has been
widely used as a fluorescent pH probe for bioimaging. The fluorescence
off–on mechanism is fundamentally important for its application and
further development. Herein, the sensing mechanism, especially the
relevant excited-state dynamics, of pHrodo is investigated by
steady-state and time-resolved spectroscopy as well as quantum chemical
calculations, showing that pHrodo is best understood using the
bichromophore model. Its first excited state (S1) is a charge transfer state between two chromophores. From S1, pHrodo relaxes to its ground state (S0) via an ultrafast nonradiative process (∼0.5 ps), which causes its fluorescence to be “off”. After protonation, S1
becomes a localized excited state, which accounts for the fluorescence
being turned “on”. Our work provides photophysical insight into the
sensing mechanism of pHrodo and indicates the bichromophore model might
be relevant to a wide range of fluorescent probes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/48445f2a-d289-4f8a-89ca-9f9875657158
- author
- Jiang, Simin ; He, Yanmei LU ; Brandt, Jonas Højberg ; Zhao, Li LU and Chen, Junsheng LU
- organization
- publishing date
- 2023-11-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Letters
- volume
- 14
- issue
- 46
- pages
- 7 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85178545707
- pmid:37967406
- ISSN
- 1948-7185
- DOI
- 10.1021/acs.jpclett.3c02653
- language
- English
- LU publication?
- yes
- id
- 48445f2a-d289-4f8a-89ca-9f9875657158
- date added to LUP
- 2024-05-03 10:25:49
- date last changed
- 2024-05-18 06:28:45
@article{48445f2a-d289-4f8a-89ca-9f9875657158, abstract = {{The pHrodo with an “off–on” response to the changes of pH has been <br> widely used as a fluorescent pH probe for bioimaging. The fluorescence <br> off–on mechanism is fundamentally important for its application and <br> further development. Herein, the sensing mechanism, especially the <br> relevant excited-state dynamics, of pHrodo is investigated by <br> steady-state and time-resolved spectroscopy as well as quantum chemical <br> calculations, showing that pHrodo is best understood using the <br> bichromophore model. Its first excited state (S<sub>1</sub>) is a charge transfer state between two chromophores. From S<sub>1</sub>, pHrodo relaxes to its ground state (S<sub>0</sub>) via an ultrafast nonradiative process (∼0.5 ps), which causes its fluorescence to be “off”. After protonation, S<sub>1</sub><br> becomes a localized excited state, which accounts for the fluorescence <br> being turned “on”. Our work provides photophysical insight into the <br> sensing mechanism of pHrodo and indicates the bichromophore model might <br> be relevant to a wide range of fluorescent probes.}}, author = {{Jiang, Simin and He, Yanmei and Brandt, Jonas Højberg and Zhao, Li and Chen, Junsheng}}, issn = {{1948-7185}}, language = {{eng}}, month = {{11}}, number = {{46}}, pages = {{10482--10488}}, publisher = {{The American Chemical Society (ACS)}}, series = {{The Journal of Physical Chemistry Letters}}, title = {{Sensing Mechanism and Excited-State Dynamics of a Widely Used Intracellular Fluorescent pH Probe : pHrodo}}, url = {{http://dx.doi.org/10.1021/acs.jpclett.3c02653}}, doi = {{10.1021/acs.jpclett.3c02653}}, volume = {{14}}, year = {{2023}}, }