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Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey

Inci, A.; Yildirim, A.; Njabo, K. Y.; Duzlu, O.; Biskin, Z. and Ciloglu, A. LU (2012) In Veterinary Parasitology 188(1-2). p.179-184
Abstract

Assessing vector-parasite relationship is important in understanding the emergence of vector-borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June-August of 2008 and 2009. Each mosquito's head-thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax-head, 599 abdomen) were constituted of which128 pools (59 thorax-head, 69 abdomen) were... (More)

Assessing vector-parasite relationship is important in understanding the emergence of vector-borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June-August of 2008 and 2009. Each mosquito's head-thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax-head, 599 abdomen) were constituted of which128 pools (59 thorax-head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax-head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cyt. b gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Avian, Molecular characterization, Plasmodium spp., Turkey, Vector
in
Veterinary Parasitology
volume
188
issue
1-2
pages
6 pages
external identifiers
  • scopus:84863228812
ISSN
0304-4017
DOI
10.1016/j.vetpar.2012.02.012
language
English
LU publication?
no
id
484d288c-faff-4c12-8a4d-7574940e8f2d
date added to LUP
2017-12-04 15:18:44
date last changed
2018-07-08 04:28:36
@article{484d288c-faff-4c12-8a4d-7574940e8f2d,
  abstract     = {<p>Assessing vector-parasite relationship is important in understanding the emergence of vector-borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June-August of 2008 and 2009. Each mosquito's head-thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax-head, 599 abdomen) were constituted of which128 pools (59 thorax-head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax-head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cyt. b gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey.</p>},
  author       = {Inci, A. and Yildirim, A. and Njabo, K. Y. and Duzlu, O. and Biskin, Z. and Ciloglu, A.},
  issn         = {0304-4017},
  keyword      = {Avian,Molecular characterization,Plasmodium spp.,Turkey,Vector},
  language     = {eng},
  month        = {08},
  number       = {1-2},
  pages        = {179--184},
  series       = {Veterinary Parasitology},
  title        = {Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey},
  url          = {http://dx.doi.org/10.1016/j.vetpar.2012.02.012},
  volume       = {188},
  year         = {2012},
}