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Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA

Kovacic, Suzana; Samii, Laleh; Lamour, Guillaume; Li, Hongbin; Linke, Heiner LU ; Bromley, Elizabeth H. C.; Woolfson, Derek N.; Curmi, Paul M. G. and Forde, Nancy R. (2014) In Biomacromolecules 15(11). p.4065-4072
Abstract
Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this... (More)
Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
15
issue
11
pages
4065 - 4072
publisher
The American Chemical Society
external identifiers
  • wos:000344721400020
  • scopus:84910069461
ISSN
1526-4602
DOI
10.1021/bm501109p
language
English
LU publication?
yes
id
01aa8e3f-e2ca-495d-9205-c18d56a939ec (old id 4865268)
date added to LUP
2014-12-18 11:22:12
date last changed
2017-03-05 03:10:22
@article{01aa8e3f-e2ca-495d-9205-c18d56a939ec,
  abstract     = {Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.},
  author       = {Kovacic, Suzana and Samii, Laleh and Lamour, Guillaume and Li, Hongbin and Linke, Heiner and Bromley, Elizabeth H. C. and Woolfson, Derek N. and Curmi, Paul M. G. and Forde, Nancy R.},
  issn         = {1526-4602},
  language     = {eng},
  number       = {11},
  pages        = {4065--4072},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA},
  url          = {http://dx.doi.org/10.1021/bm501109p},
  volume       = {15},
  year         = {2014},
}