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Gelatin-Hydroxyapatite Cryogels with Bone Morphogenetic Protein-2 and Transforming Growth Factor Beta-1 for Calvarial Defects

Inci, Ilyas LU ; Odabas, Sedat; Vargel, Ibrahim; Guzel, Elif; Korkusuz, Petek; Cavusoglu, Tarik; Kirsebom, Harald LU ; Celik, Hakan Hamdi; Demirkazik, Figen and Mattiasson, Bo LU , et al. (2014) In Journal of Biomaterials and Tissue Engineering 4(8). p.624-631
Abstract
Over recent years, several tissue engineering techniques are used for bone regeneration. In this study gelatin-hydroxyapatite (Gelatin-HA) cryogel scaffolds were used with/without bone morphogenetic protein-2 (BMP-2) and transforming growth factor beta-1 (TGF-beta 1) to heal critical sized bone defects. Release of growth factors from the cryogels were performed up to 28 days in vitro. Moreover, in vivo study were performed by 6 groups and results were evaluated by micro computerized tomography (mu-CT) and histology on 2 months and 4 months after surgery. Gene expression studies were also performed on 2 weeks and 4 weeks after surgery. Collagen type I (Col I), alkaline phosphatase (ALP) and osteocalcin (OSC) gene expression levels were... (More)
Over recent years, several tissue engineering techniques are used for bone regeneration. In this study gelatin-hydroxyapatite (Gelatin-HA) cryogel scaffolds were used with/without bone morphogenetic protein-2 (BMP-2) and transforming growth factor beta-1 (TGF-beta 1) to heal critical sized bone defects. Release of growth factors from the cryogels were performed up to 28 days in vitro. Moreover, in vivo study were performed by 6 groups and results were evaluated by micro computerized tomography (mu-CT) and histology on 2 months and 4 months after surgery. Gene expression studies were also performed on 2 weeks and 4 weeks after surgery. Collagen type I (Col I), alkaline phosphatase (ALP) and osteocalcin (OSC) gene expression levels were analyzed. According to histology and mu-CT results, there was a significant bone healing in the BMP-2 containing gelatin-HA cryogel group (Group I) as compared to other groups except autograft applied group (Group IV). Moreover, significant increases were observed for Col I, ALP and OSC in Group I and Group IV. However there was no significant increase in the group of BMP-2 and TGF-beta 1 applied together (Group II). The results reveal that combination of gelatin-HA cryogels with BMP-2 is a promising candidate for healing in critical sized craniofacial bone defects. (Less)
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Contribution to journal
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keywords
Gelatin, Hydroxyapatite, Cryogel, Bone Tissue Engineering, Bone, Morphogenetic Protein-2, Transforming Growth Factor Beta-1
in
Journal of Biomaterials and Tissue Engineering
volume
4
issue
8
pages
624 - 631
publisher
American Scientific Publishers
external identifiers
  • wos:000344575300003
  • scopus:84924694030
ISSN
2157-9091
DOI
10.1166/jbt.2014.1210
language
English
LU publication?
yes
id
612e53de-6fe1-4aef-ac51-477760668391 (old id 4871828)
date added to LUP
2015-01-07 10:58:18
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2017-08-27 03:36:39
@article{612e53de-6fe1-4aef-ac51-477760668391,
  abstract     = {Over recent years, several tissue engineering techniques are used for bone regeneration. In this study gelatin-hydroxyapatite (Gelatin-HA) cryogel scaffolds were used with/without bone morphogenetic protein-2 (BMP-2) and transforming growth factor beta-1 (TGF-beta 1) to heal critical sized bone defects. Release of growth factors from the cryogels were performed up to 28 days in vitro. Moreover, in vivo study were performed by 6 groups and results were evaluated by micro computerized tomography (mu-CT) and histology on 2 months and 4 months after surgery. Gene expression studies were also performed on 2 weeks and 4 weeks after surgery. Collagen type I (Col I), alkaline phosphatase (ALP) and osteocalcin (OSC) gene expression levels were analyzed. According to histology and mu-CT results, there was a significant bone healing in the BMP-2 containing gelatin-HA cryogel group (Group I) as compared to other groups except autograft applied group (Group IV). Moreover, significant increases were observed for Col I, ALP and OSC in Group I and Group IV. However there was no significant increase in the group of BMP-2 and TGF-beta 1 applied together (Group II). The results reveal that combination of gelatin-HA cryogels with BMP-2 is a promising candidate for healing in critical sized craniofacial bone defects.},
  author       = {Inci, Ilyas and Odabas, Sedat and Vargel, Ibrahim and Guzel, Elif and Korkusuz, Petek and Cavusoglu, Tarik and Kirsebom, Harald and Celik, Hakan Hamdi and Demirkazik, Figen and Mattiasson, Bo and Piskin, Erhan},
  issn         = {2157-9091},
  keyword      = {Gelatin,Hydroxyapatite,Cryogel,Bone Tissue Engineering,Bone,Morphogenetic Protein-2,Transforming Growth Factor Beta-1},
  language     = {eng},
  number       = {8},
  pages        = {624--631},
  publisher    = {American Scientific Publishers},
  series       = {Journal of Biomaterials and Tissue Engineering},
  title        = {Gelatin-Hydroxyapatite Cryogels with Bone Morphogenetic Protein-2 and Transforming Growth Factor Beta-1 for Calvarial Defects},
  url          = {http://dx.doi.org/10.1166/jbt.2014.1210},
  volume       = {4},
  year         = {2014},
}