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Gelatin-Modified Bone Substitute with Bioactive Molecules Enhance Cellular Interactions and Bone Regeneration

Teotia, Arun Kumar; Gupta, Ankur; Raina, Deepak Bushan LU ; Lidgren, Lars LU and Kumar, Ashok LU (2016) In ACS Applied Materials and Interfaces 8(17). p.10775-10787
Abstract

In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements... (More)

In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared to other groups.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biomimetic, bisphosphonates, calcium sulfate, hydroxyapatite, osteoblasts, osteoinductivity, osteopromotive
in
ACS Applied Materials and Interfaces
volume
8
issue
17
pages
13 pages
publisher
The American Chemical Society
external identifiers
  • scopus:84969594335
  • wos:000375520700016
ISSN
1944-8244
DOI
10.1021/acsami.6b02145
language
English
LU publication?
yes
id
4873829a-f43b-43bf-a3a5-87a40b0a70df
date added to LUP
2017-02-02 11:11:02
date last changed
2017-11-05 05:13:00
@article{4873829a-f43b-43bf-a3a5-87a40b0a70df,
  abstract     = {<p>In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared to other groups.</p>},
  author       = {Teotia, Arun Kumar and Gupta, Ankur and Raina, Deepak Bushan and Lidgren, Lars and Kumar, Ashok},
  issn         = {1944-8244},
  keyword      = {biomimetic,bisphosphonates,calcium sulfate,hydroxyapatite,osteoblasts,osteoinductivity,osteopromotive},
  language     = {eng},
  month        = {05},
  number       = {17},
  pages        = {10775--10787},
  publisher    = {The American Chemical Society},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Gelatin-Modified Bone Substitute with Bioactive Molecules Enhance Cellular Interactions and Bone Regeneration},
  url          = {http://dx.doi.org/10.1021/acsami.6b02145},
  volume       = {8},
  year         = {2016},
}