Gelatin-Modified Bone Substitute with Bioactive Molecules Enhance Cellular Interactions and Bone Regeneration
(2016) In ACS Applied Materials and Interfaces 8(17). p.10775-10787- Abstract
In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements... (More)
In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared to other groups.
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- author
- Teotia, Arun Kumar ; Gupta, Ankur ; Raina, Deepak Bushan LU ; Lidgren, Lars LU and Kumar, Ashok LU
- organization
- publishing date
- 2016-05-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- biomimetic, bisphosphonates, calcium sulfate, hydroxyapatite, osteoblasts, osteoinductivity, osteopromotive
- in
- ACS Applied Materials and Interfaces
- volume
- 8
- issue
- 17
- pages
- 13 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:84969594335
- pmid:27077816
- wos:000375520700016
- ISSN
- 1944-8244
- DOI
- 10.1021/acsami.6b02145
- language
- English
- LU publication?
- yes
- id
- 4873829a-f43b-43bf-a3a5-87a40b0a70df
- date added to LUP
- 2017-02-02 11:11:02
- date last changed
- 2024-08-24 06:14:11
@article{4873829a-f43b-43bf-a3a5-87a40b0a70df,
abstract = {{<p>In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared to other groups.</p>}},
author = {{Teotia, Arun Kumar and Gupta, Ankur and Raina, Deepak Bushan and Lidgren, Lars and Kumar, Ashok}},
issn = {{1944-8244}},
keywords = {{biomimetic; bisphosphonates; calcium sulfate; hydroxyapatite; osteoblasts; osteoinductivity; osteopromotive}},
language = {{eng}},
month = {{05}},
number = {{17}},
pages = {{10775--10787}},
publisher = {{The American Chemical Society (ACS)}},
series = {{ACS Applied Materials and Interfaces}},
title = {{Gelatin-Modified Bone Substitute with Bioactive Molecules Enhance Cellular Interactions and Bone Regeneration}},
url = {{http://dx.doi.org/10.1021/acsami.6b02145}},
doi = {{10.1021/acsami.6b02145}},
volume = {{8}},
year = {{2016}},
}