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Third Generation ATP Sensor with Enzymatic Analyte Recycling

Yarman, Aysu; Schulz, Christopher LU ; Sygmund, Cristoph; Ludwig, Roland; Gorton, Lo LU ; Wollenberger, Ulla and Scheller, Frieder W. (2014) In Electroanalysis 26(9). p.2043-2048
Abstract
For the first time the direct electron transfer of an enzyme - cellobiose dehydrogenase, CDH - has been coupled with the hexokinase catalyzed competition for glucose in a sensor for ATP. To enhance the signal output for ATP, pyruvate kinase was coimmobilized to recycle ADP by the phosphoenolpyruvate driven reaction. The new sensor overcomes the limit of 1:1 stoichiometry of the sequential or competitive conversion of ATP by effective enzymatic recycling of the analyte. The anodic oxidation of the glucose converting CDH proceeds at electrode potentials below 0 mV vs. Ag vertical bar AgCl thus potentially interfering substances like ascorbic acid or catecholamines do not influence the measuring signal. The combination of direct electron... (More)
For the first time the direct electron transfer of an enzyme - cellobiose dehydrogenase, CDH - has been coupled with the hexokinase catalyzed competition for glucose in a sensor for ATP. To enhance the signal output for ATP, pyruvate kinase was coimmobilized to recycle ADP by the phosphoenolpyruvate driven reaction. The new sensor overcomes the limit of 1:1 stoichiometry of the sequential or competitive conversion of ATP by effective enzymatic recycling of the analyte. The anodic oxidation of the glucose converting CDH proceeds at electrode potentials below 0 mV vs. Ag vertical bar AgCl thus potentially interfering substances like ascorbic acid or catecholamines do not influence the measuring signal. The combination of direct electron transfer of CDH with the enzymatic recycling results in an interference-free and oxygen-independent measurement of ATP in the lower mu molar concentration range with a lower limit of detection of 63.3 nM (S/N=3). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ATP, Third generation sensor, Enzymatic recycling, Cellobiose, dehydrogenase, Hexokinase, Pyruvate kinase
in
Electroanalysis
volume
26
issue
9
pages
2043 - 2048
publisher
John Wiley & Sons
external identifiers
  • wos:000343926700023
  • scopus:84908159418
ISSN
1040-0397
DOI
10.1002/elan.201400231
language
English
LU publication?
yes
id
4730412c-a186-4540-bca0-f77be035b8eb (old id 4875859)
date added to LUP
2014-12-30 09:46:40
date last changed
2017-08-06 04:19:10
@article{4730412c-a186-4540-bca0-f77be035b8eb,
  abstract     = {For the first time the direct electron transfer of an enzyme - cellobiose dehydrogenase, CDH - has been coupled with the hexokinase catalyzed competition for glucose in a sensor for ATP. To enhance the signal output for ATP, pyruvate kinase was coimmobilized to recycle ADP by the phosphoenolpyruvate driven reaction. The new sensor overcomes the limit of 1:1 stoichiometry of the sequential or competitive conversion of ATP by effective enzymatic recycling of the analyte. The anodic oxidation of the glucose converting CDH proceeds at electrode potentials below 0 mV vs. Ag vertical bar AgCl thus potentially interfering substances like ascorbic acid or catecholamines do not influence the measuring signal. The combination of direct electron transfer of CDH with the enzymatic recycling results in an interference-free and oxygen-independent measurement of ATP in the lower mu molar concentration range with a lower limit of detection of 63.3 nM (S/N=3).},
  author       = {Yarman, Aysu and Schulz, Christopher and Sygmund, Cristoph and Ludwig, Roland and Gorton, Lo and Wollenberger, Ulla and Scheller, Frieder W.},
  issn         = {1040-0397},
  keyword      = {ATP,Third generation sensor,Enzymatic recycling,Cellobiose,dehydrogenase,Hexokinase,Pyruvate kinase},
  language     = {eng},
  number       = {9},
  pages        = {2043--2048},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Third Generation ATP Sensor with Enzymatic Analyte Recycling},
  url          = {http://dx.doi.org/10.1002/elan.201400231},
  volume       = {26},
  year         = {2014},
}