Low salivary IgA activity to cell-surface antigens of mutans streptococci related to HLA-DRB1*04
(2005) In Oral Microbiology and Immunology 20(2). p.73-81- Abstract
- Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a... (More)
- Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a larger study group, the salivary IgA activity to antigens of three oral streptococci in relation to different HLA-DRB1*04 alleles. Methods: Stimulated saliva was collected from 58 HLA-DRB1*04-positive individuals. Whole cell antigen extracts from S. mutans, Streptococcus sobrinus and Streptococcus parasanguis and the streptococcal antigen (SA) I/II were separated in SDS-PAGE, transblotted and detected with diluted saliva (Western blot), and analyzed in a computer program. All distinct immunoblot bands over 100 kDa were recorded and compared in relation to DRB1*04. Results: The immunoblots revealed lower salivary IgA reactions to S. mutans, S. sobrinus and SA I/II, but not to S. parasanguis, for the DRB1*0401- and *0404-positive individuals compared to other DRB1*04 types. For the *0401 subgroup there was a significant association with a lower IgA response to S. mutans. Conclusion: The results confirm earlier observations and may also support previous demonstrated association between colonization by mutans streptococci and the serologically defined HLA-DR4. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/253464
- author
- Wallengren, MLL ; Hamberg, K ; Ericson, D and Nordberg, Jonas LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- salivary IgA, oral streptococci, cell-surface antigen, HLA, Western blot
- in
- Oral Microbiology and Immunology
- volume
- 20
- issue
- 2
- pages
- 73 - 81
- publisher
- Wiley
- external identifiers
-
- pmid:15720566
- wos:000227130300002
- scopus:14944342448
- ISSN
- 0902-0055
- DOI
- 10.1111/j.1399-302X.2004.00192.x
- language
- English
- LU publication?
- yes
- id
- 48952bb5-4d34-4684-b45b-6162db65fddf (old id 253464)
- date added to LUP
- 2016-04-01 16:22:53
- date last changed
- 2022-01-28 19:17:46
@article{48952bb5-4d34-4684-b45b-6162db65fddf,
abstract = {{Background/aims: Mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. These differences are not readily explained, though several factors are believed to influence the colonization. One factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin A (IgA). In a previous study, differences in salivary IgA reactions to oral streptococci were observed between human leukocyte antigen (HLA)-DR4-positive and DR4-negative individuals. A lower salivary IgA activity to Streptococcus mutans in particular was most pronounced for two DR4 subgroups, DRB1*0401 and *0404. The main purpose of this study was to further investigate, in a larger study group, the salivary IgA activity to antigens of three oral streptococci in relation to different HLA-DRB1*04 alleles. Methods: Stimulated saliva was collected from 58 HLA-DRB1*04-positive individuals. Whole cell antigen extracts from S. mutans, Streptococcus sobrinus and Streptococcus parasanguis and the streptococcal antigen (SA) I/II were separated in SDS-PAGE, transblotted and detected with diluted saliva (Western blot), and analyzed in a computer program. All distinct immunoblot bands over 100 kDa were recorded and compared in relation to DRB1*04. Results: The immunoblots revealed lower salivary IgA reactions to S. mutans, S. sobrinus and SA I/II, but not to S. parasanguis, for the DRB1*0401- and *0404-positive individuals compared to other DRB1*04 types. For the *0401 subgroup there was a significant association with a lower IgA response to S. mutans. Conclusion: The results confirm earlier observations and may also support previous demonstrated association between colonization by mutans streptococci and the serologically defined HLA-DR4.}},
author = {{Wallengren, MLL and Hamberg, K and Ericson, D and Nordberg, Jonas}},
issn = {{0902-0055}},
keywords = {{salivary IgA; oral streptococci; cell-surface antigen; HLA; Western blot}},
language = {{eng}},
number = {{2}},
pages = {{73--81}},
publisher = {{Wiley}},
series = {{Oral Microbiology and Immunology}},
title = {{Low salivary IgA activity to cell-surface antigens of mutans streptococci related to HLA-DRB1*04}},
url = {{http://dx.doi.org/10.1111/j.1399-302X.2004.00192.x}},
doi = {{10.1111/j.1399-302X.2004.00192.x}},
volume = {{20}},
year = {{2005}},
}