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Improved propionic acid production from glycerol: combining cyclic batch-and sequential batch fermentations with optimal nutrient composition

Dishisha, Tarek LU ; Ibrahim, Mohammad H A LU ; Alvarez Aliaga, Teresa LU ; Cavero, Hugo LU and Hatti-Kaul, Rajni LU (2015) In Bioresource Technology 176. p.80-87
Abstract
Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (w w−1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93 mol% and productivity of... (More)
Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (w w−1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93 mol% and productivity of 0.53 g L−1 h−1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1 g L−1 h−1 at initial glycerol up to 120 g L−1, and a maximum of 1.63 g L−1 h−1 from 90 g L−1 glycerol. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Propionic acid, Propionibacterium acidipropionici, Carbon:nitrogen ratio, Cyclic batch fermentation, Sequential batch fermentation
in
Bioresource Technology
volume
176
pages
80 - 87
publisher
Elsevier
external identifiers
  • pmid:25460987
  • wos:000345982900011
  • scopus:84911417657
ISSN
1873-2976
DOI
10.1016/j.biortech.2014.11.013
language
English
LU publication?
yes
id
a340dd2e-fd45-45e2-8b88-a57a47cf6466 (old id 4897180)
date added to LUP
2015-01-12 13:24:40
date last changed
2017-10-22 03:23:20
@article{a340dd2e-fd45-45e2-8b88-a57a47cf6466,
  abstract     = {Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (w w−1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93 mol% and productivity of 0.53 g L−1 h−1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1 g L−1 h−1 at initial glycerol up to 120 g L−1, and a maximum of 1.63 g L−1 h−1 from 90 g L−1 glycerol.},
  author       = {Dishisha, Tarek and Ibrahim, Mohammad H A and Alvarez Aliaga, Teresa and Cavero, Hugo and Hatti-Kaul, Rajni},
  issn         = {1873-2976},
  keyword      = {Propionic acid,Propionibacterium acidipropionici,Carbon:nitrogen ratio,Cyclic batch fermentation,Sequential batch fermentation},
  language     = {eng},
  pages        = {80--87},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Improved propionic acid production from glycerol: combining cyclic batch-and sequential batch fermentations with optimal nutrient composition},
  url          = {http://dx.doi.org/10.1016/j.biortech.2014.11.013},
  volume       = {176},
  year         = {2015},
}