Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata
(2010) In NMR in Biomedicine 23(9). p.102-1097- Abstract
The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE = 2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE = 2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE = 20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the... (More)
The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE = 2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE = 2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE = 20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25%. Absolute concentrations of metabolites in MO, such as glutamate, glutamine, γ-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts. Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p < 0.001) concentrations of glutamate (-50 ± 4%), glutamine (-54 ± 3%) and taurine (-78 ± 3%). This study suggests that the functional specialisation of distinct brain regions is reflected in the neurochemical profile.
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- author
- Xin, Lijing ; Gambarota, Giulio ; Duarte, João M N LU ; Mlynárik, Vladimír and Gruetter, Rolf
- publishing date
- 2010-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Cerebral Cortex, Corpus Striatum, Glycine, Hippocampus, Humans, Magnetic Resonance Spectroscopy, Medulla Oblongata, Rats, Rats, Sprague-Dawley, Journal Article, Research Support, Non-U.S. Gov't
- in
- NMR in Biomedicine
- volume
- 23
- issue
- 9
- pages
- 102 - 1097
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:20963803
- scopus:79251514306
- ISSN
- 0952-3480
- DOI
- 10.1002/nbm.1537
- language
- English
- LU publication?
- no
- id
- 48a88c15-718f-4694-b3fe-5eb8d1cbe164
- date added to LUP
- 2017-10-19 15:19:59
- date last changed
- 2024-09-17 10:21:09
@article{48a88c15-718f-4694-b3fe-5eb8d1cbe164, abstract = {{<p>The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE = 2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE = 2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE = 20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25%. Absolute concentrations of metabolites in MO, such as glutamate, glutamine, γ-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts. Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p < 0.001) concentrations of glutamate (-50 ± 4%), glutamine (-54 ± 3%) and taurine (-78 ± 3%). This study suggests that the functional specialisation of distinct brain regions is reflected in the neurochemical profile.</p>}}, author = {{Xin, Lijing and Gambarota, Giulio and Duarte, João M N and Mlynárik, Vladimír and Gruetter, Rolf}}, issn = {{0952-3480}}, keywords = {{Animals; Cerebral Cortex; Corpus Striatum; Glycine; Hippocampus; Humans; Magnetic Resonance Spectroscopy; Medulla Oblongata; Rats; Rats, Sprague-Dawley; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{9}}, pages = {{102--1097}}, publisher = {{John Wiley & Sons Inc.}}, series = {{NMR in Biomedicine}}, title = {{Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata}}, url = {{http://dx.doi.org/10.1002/nbm.1537}}, doi = {{10.1002/nbm.1537}}, volume = {{23}}, year = {{2010}}, }