Caracterización molecular del CVA24v aislado en Cuba duran-te 23 años, 1986-2003, revela hallazgos en la epidemiología y patogenia del virus
(2023) In Anales de la Academia de Ciencias de Cuba 13(3).- Abstract
Introduction: The CVA24 antigenic variant is the causal agent of epidemics of acute hemorrhagic conjunctivitis that has prevailed to date. To study the transmission dynamics and evolution of this variant, we molecularly characterized Cuban isolates obtained in 5 epidemic periods between 1986 and 2009. Methods: Using the BEAST v1.8.4 program, it was performed the phylogenetic and phylodynamic analysis of the partial genomic regions of 54 Cuban sequences and 83 sequences from 17 countries for the 3C genomic region and 35 Cuban sequences with 95 sequences from 18 countries for the VP1 region. They were analyzed the amino acid changes of the Cuban sequences and they were compared the sequences obtained from conjunctival exudates and feces.... (More)
Introduction: The CVA24 antigenic variant is the causal agent of epidemics of acute hemorrhagic conjunctivitis that has prevailed to date. To study the transmission dynamics and evolution of this variant, we molecularly characterized Cuban isolates obtained in 5 epidemic periods between 1986 and 2009. Methods: Using the BEAST v1.8.4 program, it was performed the phylogenetic and phylodynamic analysis of the partial genomic regions of 54 Cuban sequences and 83 sequences from 17 countries for the 3C genomic region and 35 Cuban sequences with 95 sequences from 18 countries for the VP1 region. They were analyzed the amino acid changes of the Cuban sequences and they were compared the sequences obtained from conjunctival exudates and feces. Results: It was identified that each epidemic period was caused by different genetic variants, and homologous to variants that circulated in the Americas, Africa, and Asia, and that could circulate up to 2 years prior to the epidemic peaks. The virus showed a stable effective population over time, increasing in pandemic periods, with a nucleotide substitution rate (substitutions/site/year) of 4.39 × 10-3 for the 3C region and 5.80 × 10-3 for VP1. They were obtained 19 viral transmission routes for the 3C genomic region and 25 routes for VP1. They were inferred the routes of introduction of the virus to Cuba in the epidemic periods of the years 1986, 1997, 2003 and 2008-2009 by the ancestral relationships of the Cuban sequences with sequences of destination countries of some routes, endorsed by viral transmission networks. Amino acid changes in both genomic regions could be involved in the evolution of CVA24v and it was obtained evidence that supports the use of feces to identify this viral variant. The conclusions were new knowledge in the molecular epidemiology of CVA24v that circulated in Cuba and the world for 23 years after its global emergence.
(Less)
- author
- Fonseca Quintana, Magilé C. ; Pupo Meriño, Mario ; Sarmiento Pérez, Luis R. LU ; García González, Luis A. ; Resik, Sonia ; Heng Hung, Lai and Muné, Mayra
- organization
- alternative title
- Molecular characterization of CV A24v in Cuba over 23-years, 1986-2009, reveals findings in the epidemiology and pathoge-nesis of the virus
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 3C protease, acute hemorrhagic conjunctivitis, Coxsackievirus A24 variant, phylodynamic, phylogeny, VP1
- in
- Anales de la Academia de Ciencias de Cuba
- volume
- 13
- issue
- 3
- article number
- e1345
- publisher
- Academia de Ciencias de Cuba
- external identifiers
-
- scopus:85193776026
- ISSN
- 2304-0106
- language
- Spanish
- LU publication?
- yes
- id
- 48aad7cd-f578-4576-9032-3cd00d8d6277
- date added to LUP
- 2024-06-18 14:16:35
- date last changed
- 2024-06-19 09:22:21
@article{48aad7cd-f578-4576-9032-3cd00d8d6277,
abstract = {{<p>Introduction: The CVA24 antigenic variant is the causal agent of epidemics of acute hemorrhagic conjunctivitis that has prevailed to date. To study the transmission dynamics and evolution of this variant, we molecularly characterized Cuban isolates obtained in 5 epidemic periods between 1986 and 2009. Methods: Using the BEAST v1.8.4 program, it was performed the phylogenetic and phylodynamic analysis of the partial genomic regions of 54 Cuban sequences and 83 sequences from 17 countries for the 3C genomic region and 35 Cuban sequences with 95 sequences from 18 countries for the VP1 region. They were analyzed the amino acid changes of the Cuban sequences and they were compared the sequences obtained from conjunctival exudates and feces. Results: It was identified that each epidemic period was caused by different genetic variants, and homologous to variants that circulated in the Americas, Africa, and Asia, and that could circulate up to 2 years prior to the epidemic peaks. The virus showed a stable effective population over time, increasing in pandemic periods, with a nucleotide substitution rate (substitutions/site/year) of 4.39 × 10<sup>-3</sup> for the 3C region and 5.80 × 10<sup>-3</sup> for VP1. They were obtained 19 viral transmission routes for the 3C genomic region and 25 routes for VP1. They were inferred the routes of introduction of the virus to Cuba in the epidemic periods of the years 1986, 1997, 2003 and 2008-2009 by the ancestral relationships of the Cuban sequences with sequences of destination countries of some routes, endorsed by viral transmission networks. Amino acid changes in both genomic regions could be involved in the evolution of CVA24v and it was obtained evidence that supports the use of feces to identify this viral variant. The conclusions were new knowledge in the molecular epidemiology of CVA24v that circulated in Cuba and the world for 23 years after its global emergence.</p>}},
author = {{Fonseca Quintana, Magilé C. and Pupo Meriño, Mario and Sarmiento Pérez, Luis R. and García González, Luis A. and Resik, Sonia and Heng Hung, Lai and Muné, Mayra}},
issn = {{2304-0106}},
keywords = {{3C protease; acute hemorrhagic conjunctivitis; Coxsackievirus A24 variant; phylodynamic; phylogeny; VP1}},
language = {{spa}},
number = {{3}},
publisher = {{Academia de Ciencias de Cuba}},
series = {{Anales de la Academia de Ciencias de Cuba}},
title = {{Caracterización molecular del CVA24v aislado en Cuba duran-te 23 años, 1986-2003, revela hallazgos en la epidemiología y patogenia del virus}},
volume = {{13}},
year = {{2023}},
}